It appears that your browser has JavaScript disabled.

This Website requires your browser to be JavaScript enabled.

Please enable JavaScript and reload this page.

Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.

MAB3747 Sigma-AldrichAnti-Microphthalmia (Mi) Antibody, clone C5

Anti-Microphthalmia (Mi) Antibody, clone C5 is a Mouse Monoclonal Antibody for detection of Microphthalmia (Mi) also known as Microphthalmia-associated transcription factor & has been tested in EMSA, IHC, IHC(P), IP & WB.

Anti-Microphthalmia (Mi) Antibody, clone C5 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

FDS
CoA
Références bibliographiques

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: LZMS, MCOPS1, MAA

Produits recommandés

Aperçu

Replacement Information

Offres spéciales

100% Performance Guaranteed

Tableau de caractéristiques principal

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R, M	EMSA, IHC, IH(P), IP, WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MAB3747
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Microphthalmia (Mi) Antibody, clone C5
Background Information	MiTF (Microphthalmia associated transcription factor) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, mast cells and bone development. Mutations in MiTF cause auditory pigmentary syndromes, such as Waardenburg syndrome type II, type IIa and Tietz syndrome in humans. There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteopetrotic rat strain harbors a large genomic deletion encompassing the 3' half of Mi including most of the b-HLH-ZIP region. Osteoclasts from these animals lack Mi protein in contrast to wild-type rat, mouse, and human osteoclasts.

References

Product Information
Format	Purified
HS Code	3002 15 90
Control	501 Mel human melanoma cells, wild-type human, rat, mouse osteoclast cells.
Presentation	Purified mouse monoclonal IgG1 in phosphate buffered saline with 0.08% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Microphthalmia (Mi) Antibody, clone C5 is a Mouse Monoclonal Antibody for detection of Microphthalmia (Mi) also known as Microphthalmia-associated transcription factor & has been tested in EMSA, IHC, IHC(P), IP & WB.
Key Applications	Electrophoretic Mobility Shift Assay Immunohistochemistry Immunohistochemistry (Paraffin) Immunoprecipitation Western Blotting
Application Notes	Immunoprecipitation: A previous lot of this antibody was used in IP. (2 µg/mg of protein lysate) Gel supershift assays: A previous lot of this antibody was used in EMSA. Immunohistochemistry(paraffin): A previous lot of this antibody was used in IH on frozen and formalin/paraffin tissue sections. Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells.
Clone	C5
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	In Western blotting, it recognizes a doublet of 52-56kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi). There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells andheart. It reacts with both melanocytic as well as the nonmelanocytic isoforms of Mi. This Ab does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay.
Isotype	IgG1
Species Reactivity	Human Rat Mouse
Species Reactivity Note	Expected to react with human and rat based on sequence homology.
Antibody Type	Monoclonal Antibody
Gene Symbol	LZMS MCOPS1 MAA
Purification Method	Protein A Purfied
Molecular Weight	53-56 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on Mouse Brain lysates. Western Blot Analysis: 1:500 dilution of this lot detected Mi on 10 μg of Mouse Brain lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG1 and affect product performance.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Référence	GTIN
MAB3747	04053252501678

Documentation

Anti-Microphthalmia (Mi) Antibody, clone C5 FDS

Titre
Fiche de données de sécurité des matériaux (FDS)

Anti-Microphthalmia (Mi) Antibody, clone C5 Certificats d'analyse

Titre	Numéro de lot
Anti-Microphthalmia (Mi), clone C5	3082557
Anti-Microphthalmia (Mi), clone C5 - 2397033	2397033
Anti-Microphthalmia (Mi), clone C5 - 2037527	2037527
Anti-Microphthalmia (Mi), clone C5 - 2061433	2061433
Anti-Microphthalmia (Mi), clone C5 - 2198942	2198942
Anti-Microphthalmia (Mi), clone C5 - 2293865	2293865
Anti-Microphthalmia (Mi), clone C5 - 2491103	2491103
Anti-Microphthalmia (Mi), clone C5 - 3023021	3023021
Anti-Microphthalmia (Mi), clone C5 - 3250325	3250325
Anti-Microphthalmia (Mi), clone C5 - 3301944	3301944

Références bibliographiques

Aperçu de la référence bibliographique	Nº PubMed
Partially purified components of Nardostachys chinensis suppress melanin synthesis through ERK and Akt signaling pathway with cAMP down-regulation in B16F10 cells. Ji Yeon Jang,Ha Neui Kim,Yu Ri Kim,Woo Young Choi,Yung Hyun Choi,Hwa Kyoung Shin,Byung Tae Choi Journal of ethnopharmacology 137 2010 Afficher le résumé Ethnopharmacological relevance Nardostachys chinensis has been used in folk medicine to treat melasma and lentigines in Korea. We investigated the inhibitory activities of Nardostachys chinensis in melanogenesis and its related signaling pathway.	21816215
Inhibitory effects of 1-O-methyl-fructofuranose from Schisandra chinensis fruit on melanogenesis in B16F0 melanoma cells. Eun Young Oh,Ji Yeon Jang,Yung Hyun Choi,Young Whan Choi,Byung Tae Choi Journal of ethnopharmacology 132 2009 Afficher le résumé 1-O-methyl-fructofuranose (1-O-MFF) from the fruit of Schisandra chinensis is a traditional Korean medicinal herb that has a variety of beneficial properties. The effect of purified 1-O-MFF on melanogenesis including the activation of related signaling pathways was investigated.	20723590
MAP kinase links the transcription factor Microphthalmia to c-Kit signalling in melanocytes. Hemesath, T J, et al. Nature, 391: 298-301 (1998) 1998 Afficher le résumé Germline mutations at loci encoding the transcription factor Microphthalmia (Mi), the cytokine receptor c-Kit, or its ligand Steel factor (S1) result in strikingly similar defects in mast cell and melanocyte development. Here we describe a biochemical link between Kit signalling and the activity of Mi. Stimulation of melanoma cells with S1 results in activation of MAP kinase, which in turn phosphorylates Mi at a consensus target serine. This phosphorylation upregulates Mi transactivation of the tyrosinase pigmentation gene promoter. In addition to modulating pigment production, such signalling may regulate the expression of genes essential for melanocyte survival and development. The pathway represents a new application of the general MAP kinase machinery in transducing a signal between a tissue-specific receptor at the cell surface and a tissue-specific transcription factor in the nucleus.	9440696

Produits & Applications associés

Catégories

Life Science Research > Antibodies and Assays > Primary Antibodies

Récemment consultés