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MABN2397 Anti-MYCBP2 Antibody, clone PM-8A9

MABN2397
100 μg  
Purchase on Sigma-Aldrich

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Replacement Information

Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2397
DescriptionAnti-MYCBP2 Antibody, clone PM-8A9
Alternate Names
  • E3 ubiquitin-protein ligase MYCBP2
  • Myc-binding protein 2
  • Pam/highwire/rpm-1 protein
  • Protein associated with Myc
Background InformationE3 ubiquitin-protein ligase MYCBP2 (UniProt: O75592; also known as Myc-binding protein 2, Pam/highwire/rpm-1 protein, Protein associated with Myc, MYCBP2) is encoded by the MYCBP2 (also known as KIAA0916, PAM) gene (Gene ID: 23077) in human.MYCBP2 protein mediates the ubiquitination and subsequent proteasomal degradation of TSC2/tuberin. It is ubiquitously expressed, but brain and thymus display exceptionally abundant expression. It is shown to co-localize with TSC1 and TSC2 along the neurites and in the growth cones. MYCBP2 may also function as a facilitator or regulator of transcriptional activation by MYC and have a role during synaptogenesis. It is reported to negatively regulate neuronal growth, synaptogenesis, and synaptic strength. Mice with MYCBP2-deficiency in peripheral sensory neurons show prolonged thermal hyperalgesia. Loss of MYCBP2 is also linked to constitutively activated p38 MAPK and increased expression of several proteins involved in receptor trafficking. Down-regulation of MYCBP2 in the spinal cord of adult rats is shown to enhance their. MYCBP2 is also shown to be an inhibitor of adenylyl cyclase. (Ref.: Holland, S et al. (2011). J. Biol. Chem. 286(5): 3671-3680).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MYCBP2 Antibody, clone PM-8A9, Cat. No. MABN2397, is a highly specific mouse monoclonal antibody that targets MYCBP2 (PAM) and has been tested in Immunoprecipitation and Western blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A representative lot detected MYCBP2 (Courtesy of Roberta L. Beauchamp, Massachusetts General Hospital, Boston, USA).

Western Blotting Analysis: A representative lot detected endogenous PAM (MYCBP2)and overexpressed Myc-tagged PAM (Courtesy of Roberta L. Beauchamp, Massachusetts General Hospital, Boston, USA).
Biological Information
ImmunogenMBP (Maltose-binding protein)-tagged recombinat fragment of 150 amino acids from the N-terminal region of human MYCBP2.
EpitopeN-terminus
ClonePM-8A9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone PM-8A9 specifically detects MYCBP2 in HEK293 cells. It targets an epitope within the first 150 amino acids in the N-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MYCBP2
  • KIAA0916
  • PAM
Purification MethodProtein G purified
UniProt Number
Molecular Weight~460 kDa observed; 510.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MYCBP2 in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABN2397 04054839176449

Documentation

Anti-MYCBP2 Antibody, clone PM-8A9 FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-MYCBP2 Antibody, clone PM-8A9 Certificats d'analyse

TitreNuméro de lot
Anti-MYCBP2, clone PM-8A9 - 3174764 3174764
Anti-MYCBP2, clone PM-8A9 - 3707311 3707311
Anti-MYCBP2, clone PM-8A9 - 3860860 3860860
Anti-MYCBP2, clone PM-8A9 - 4013363 4013363
Anti-MYCBP2, clone PM-8A9 - 4092647 4092647
Anti-MYCBP2, clone PM-8A9 Monoclonal Antibody 3063809
Anti-MYCBP2, clone PM-8A9 Monoclonal Antibody Q2829082