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MABE340-AF488 Anti-MDM2 Antibody, clone IF2, Alexa Fluor® 488 Conjugate

This Anti-MDM2 Antibody, clone IF2, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of MDM2.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Apoptosis - Additional Gene Symbol: MDM2 UniProt Number: Q00987
MABE340-AF488
100 µL  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberMABE340-AF488
DescriptionAnti-MDM2 Antibody, clone IF2, Alexa Fluor® 488 Conjugate
Alternate Names
  • E3 ubiquitin-protein ligase Mdm2
  • Double minute 2 protein
  • Hdm2
  • Oncoprotein Mdm2
  • p53-binding protein Mdm2
Background InformationE3 ubiquitin-protein ligase Mdm2 (UniProt Q00987; also known as Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2) is encoded by the MDM2 gene (Gene ID 4193) in human. MDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor. MDM2 antagonizes p53 transcriptional activity by binding to and blocking p53 N-terminal trans-activation domain. MDM2 can also monoubiquitinate p53, thereby promoting p53 cytoplasmic localization to prevent p53 nuclear funtion. In addition, MDM2, when in complex with p300, also mediates p53 polyubiquitination, resulting in p53 proteosomal degradation. MDM2 overexpression is shown to inhibit DNA double-strand break repair mediated through its interaction with Nbs1 in a p53-independent manner.
References
Product Information
FormatAlexaFluor®488
PresentationPurified mouse monoclonal IgG2b antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-MDM2 Antibody, clone IF2, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of MDM2.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MDM2 in A431 cells.
The unconjugated antibody (Cat. Nos. MABE340 & OP46) is shown to be suitable also for immunohistochemistry and Western blotting applications.
Biological Information
ImmunogenRecombinant human MDM2.
EpitopeWithin amino acids 26-169.
CloneIF2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone IF2 recognizes the ~85/90 kDa (apparent MW) MDM2, as well as isoforms of ~57/60 kDa and ~74/76 kDa by immunoblotting using the unconjugated antibody (Cat. Nos. MABE340 & OP46).
IsotypeIgG2b
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MDM2
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~85 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MDM2 in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABE340-AF488 04055977352863