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ABE2604 Anti-MCM5

Anti-MCM5, Cat. No. ABE2604, is a highly specific rabbit polyclonal antibody that targets DNA replication licensing factor MCM5 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-MCM5 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Gene Symbol: MCM5, CDC46 UniProt Number: P33992
ABE2604
100 µL  
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Replacement Information

Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, XnIH(P), WBRbUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABE2604
DescriptionAnti-MCM5
Alternate Names
  • DNA replication licensing factor MCM5
  • CDC46 homolog
  • P1-CDC46
Background InformationDNA replication licensing factor MCM5 (UniProt: P33992; also known as EC:3.6.4.12, CDC46 homolog, P1-CDC46) is encoded by the MCM5 (also known as CDC46) gene (Gene ID: 4174) in human. MCM5 protein as a component of the MCM2-7 (Mini-Chromosome Maintenance; MCM) complex), which consists of six sequence-related AAA + type ATPases/helicases that form a hetero-hexameric ring. It is a putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. MCM5 is upregulated in the transition from the G0 to G1/S phase of the cell cycle, indicating its active participation in cell cycle regulation. In the G1 phase, inactive MCM2-7 complex is loaded onto origins of DNA replication and during G1-S phase, MCM2-7 complex is activated to unwind the double stranded DNA and plays an important role in DNA replication forks elongation.
References
Product Information
FormatUnpurified
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MCM5, Cat. No. ABE2604, is a highly specific rabbit polyclonal antibody that targets DNA replication licensing factor MCM5 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MCM5 in human testis and human cervical cancer tissues.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MCM5 in HeLa & CHO cell lysates (Wong, P.G., et. al. (2011). PLoS One. 6(3):e17533; Borysov, S.I., et. al. (2015). Mol Cell Biol. 36(5):832-45).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MCM5 in 10 µg of MCF7 cell lysate.
Biological Information
ImmunogenA His-tagged full-length recombinant human DNA replication licensing factor MCM5.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects DNA replication licensing factor MCM5 in multiple types of human tissue sections.
IsotypeIgG
Species Reactivity
  • Human
  • Xenopus
Species Reactivity NoteHuman, Xenopus
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MCM5
  • CDC46
Purification MethodUnpurified
UniProt Number
Molecular Weight~90 kDa observed; 82.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human tonsil tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected MCM5 in human tonsil tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
ABE2604 04054839201400