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05-157-I-25UL Anti-MAPK2 (ERK2) Antibody, clone 1B3B9

05-157-I-25UL
25 µL  
Purchase on Sigma-Aldrich

Aperçu

Replacement Information
Description
Catalogue Number05-157-I-25UL
DescriptionAnti-MAPK2 (ERK2) Antibody, clone 1B3B9
Alternate Names
  • Mitogen-activated protein kinase 1
  • EC:2.7.11.24
  • MAP kinase 1
  • MAPK 1
  • ERT1
  • Extracellular signal-regulated kinase 2
  • ERK-2
  • MAP kinase isoform p42
  • p42-MAPK
  • Mitogen-activated protein kinase 2
  • MAP kinase 2
Background InformationMitogen-activated protein kinase 1 (UniProt: P63085; also known as EC:2.7.11.24; also known as MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2) is encoded by the MAPK1 (also known as ERK2, PRKM1, PRKM2) gene (Gene ID: 26413) in murine species. ERK-2 is a widely expressed proline-directed serine/threonine protein kinase that is an essential component of the MAP kinase signal transduction pathway. Its protein kinase domain is localized to amino acids 23-311. ERK-2 binds both upstream activators and downstream substrates in multimolecular complexes. Its substrates include multiple transcription factors, cytoskeletal elements, regulators of apoptosis, and regulators of translation, and signaling molecules. MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. ERK-2 is phosphorylated by MEK1 and MEK2 on threonine 183 and tyrosine 185 in the conserved TEY (Thr-Glu-Tyr) motif in response to external stimuli like insulin or NGF. Both phosphorylations are required for its activity. This phosphorylation causes conformational changes that enable full activation and interaction with its substrates.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-MAPK2 (ERK2), clone 1B3B9, Cat. No. 05-157-I, is a mouse monoclonal antibody that detects MAPK2 (ERK2) and is tested for use in Immunoprecipitation and Western Blotting.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated MAPK2 (ERK2) in Immunoprecipitation applications (Kumar, N.V., et. al. (2001). J Biol Chem. 276(34):32362-72).

Western Blotting Analysis: A representative lot detected MAPK2 (ERK2) in Western blotting applications (Sato, Y., et. al. (2001). J Biol Chem.276(15):11956-62; Wu, C., et. al. (2001). J Neurosci. 21(15):5406-16; Yamaguchi, Y., et. al. (2001). J Biol Chem. 276(22):18977-83; Utani, A., et. al. (2003). J Biol Chem. 278(36):34483-90).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHPLC purified recombinant mouse p42MAP kinase (ERK2).
EpitopeUnknown
Clone1B3B9
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1B3B9 is a mouse monoclonal antibody that recognizes MAPK2.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Rat
  • Mouse
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MAPK1
  • ERK2
  • PRKM1
  • PRKM2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~40 kDa observed; 41.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MAPK2 (ERK2) in A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
05-157-I-25UL 04061842592012

Documentation

Anti-MAPK2 (ERK2) Antibody, clone 1B3B9 FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-MAPK2 (ERK2) Antibody, clone 1B3B9 Certificats d'analyse

TitreNuméro de lot
Anti-MAPK2 (ERK2), clone 1B3B9 - Q3504043 Q3504043

Références bibliographiques

Aperçu de la référence bibliographiqueApplicationNº PubMed
Laminin alpha 3 LG4 module induces matrix metalloproteinase-1 through mitogen-activated protein kinase signaling
Atsushi Utani 1 , Yutaka Momota, Hideharu Endo, Yoshitoshi Kasuya, Konrad Beck, Nobuharu Suzuki, Motoyoshi Nomizu, Hiroshi Shinkai
J Biol Chem  278(36)  34483-90  2003

Afficher le résumé
Immunoblotting (Western)12826666 12826666
Overexpression of N-acetylglucosaminyltransferase III enhances the epidermal growth factor-induced phosphorylation of ERK in HeLaS3 cells by up-regulation of the internalization rate of the receptors
Y Sato 1 , M Takahashi, Y Shibukawa, S K Jain, R Hamaoka, Miyagawa Ji, Y Yaginuma, K Honke, M Ishikawa, N Taniguchi
J Biol Chem  276(15)  11956-62  2001

Afficher le résumé
Immunoblotting (Western)11134020 11134020
RhoA inhibits the nerve growth factor-induced Rac1 activation through Rho-associated kinase-dependent pathway
Y Yamaguchi 1 , H Katoh, H Yasui, K Mori, M Negishi
J Biol Chem  276(22)  18977-83  2001

Afficher le résumé
Immunoblotting (Western)11279039 11279039
Nerve growth factor activates persistent Rap1 signaling in endosomes
C Wu 1 , C F Lai, W C Mobley
J Neurosci  21(15)  5406-16  2001

Afficher le résumé
Immunoblotting (Western), Immunoprecipitation11466412 11466412
Ten ERK-related proteins in three distinct classes associate with AP-1 proteins and/or AP-1 DNA
N V Kumar 1 , L R Bernstein
J Biol Chem  276(34)  32362-72  2001

Afficher le résumé
Kinase Assay11431474 11431474