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MABN2614-25UL Anti-LOTUS Antibody, clone H24G11

Anti-LOTUS, clone H24G11, Cat. No. MABN2614, is a hamster monoclonal antibody that detects Cartilage acidic protein 1 (LOTUS) and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

MABN2614-25UL
25 µL  
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Replacement Information
Description
Catalogue NumberMABN2614-25UL
DescriptionAnti-LOTUS Antibody, clone H24G11
Alternate Names
  • Cartilage acidic protein 1
  • 68 kDa chondrocyte-expressed protein
  • CEP-68
  • ASPIC
  • Protein CRTAC1-B
Background InformationCartilage acidic protein 1 (UniProt: Q8R555; also known as 68 kDa chondrocyte-expressed protein, CEP-68, ASPIC, Protein CRTAC1-B, Lateral olfactory tract usher substance, LOTUS) is encoded by the Crtac1 (also known as Aspic1, Cep68) gene (Gene ID: 72832) in murine species. LOTUS is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to generate the mature form (aa 29-646). It is a membrane-bound and/or secreted protein that serves as an endogenous Nogo receptor-1 (NgR1) antagonist. It is shown to contribute to the formation of the lateral olfactory tract (LOT) axonal bundle. Endogenous Nogo-A signaling through NgR1 is shown to induce axonal branching. Developmental up-regulation of Nogo-A promotes collateral formation of the LOT during the late development period. It has been reported that axon growth inhibitors, which include Nogo proteins, myelin-associated glycoprotein (MAG), oligodendrocyte myelin glycoprotein (OMgp), and B lymphocyte stimulator (BLyS), bind to NgR1 and restrict functional recovery after damage to the adult central nervous system. LOTUS blocks the binding of these ligands to NgR1 and suppresses growth cone collapse. LOTUS knockout mice show significantly increased collateral branches of axons in the LOT. On the other hand, this branching is diminished in NgR1 knockout mice. A simultaneous loss of LOTUS and NgR1 is reported to rescue the abnormal increase in axonal branches. (Ref.: Iketani, M., et al. (2016). Sci. Rep. 6; 39586; Kurihara, Y., et al. (2014). Mol. Cell. Neurosci. 61; 211-218).
References
Product Information
FormatPurified
PresentationPurified hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-LOTUS, clone H24G11, Cat. No. MABN2614, is a hamster monoclonal antibody that detects Cartilage acidic protein 1 (LOTUS) and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunocytochemistry
Application NotesTested Applications

Immunocytochemistry Analysis: A representative lot detected LOTUS in Immunocytochemistry applications (Kurihara, Y., et al. (2014). Mol Cell Neurosci. 61:211-8; Sato, Y., et al. (2011). Science. 333(6043):769-73).

Immunohistochemistry Applications: A 1:50 dilutions from a representative lot detected LOTUS in E14 embryonic mouse tissue sections.

Western Blotting Analysis: A representative lot detected LOTUS in Western Blotting applications (Iketani, M., et al. (2016). Sci Rep. 6:39586).

Immunohistochemistry Applications: A representative lot detected LOTUS in Immunohistochemistry applications ( Sato, Y., et al. (2011). Science. 333(6043):769-73; Iketani, M., et al. (2016). Sci Rep. 6:39586).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHomogenate of protein extract from developing lateral olfactory tract and the surrounding tissues of mouse.
EpitopeUnknown
CloneH24G11
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostHamster
SpecificityClone H24G11 is a hamster monoclonal antibody that detects murine cartilage acidic protein 1B (LOTUS).
IsotypeIgGκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Crtac1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~85 kDa observed; 70.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Mouse E14 brain tissue lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected LOTUS in Mouse E14 brain tissue lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABN2614-25UL 04065266876215