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ABC232 Anti-LC3 Antibody

ABC232
100 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWBRbSerumPolyclonal Antibody
Description
Catalogue NumberABC232
ReplacesMABC177; MABC176
DescriptionAnti-LC3 Antibody
Alternate Names
  • Microtubule-associated proteins 1A/1B light chain 3A
  • Autophagy-related protein LC3 A
  • Autophagy-related ubiquitin-like modifier LC3 A
  • MAP1 light chain 3-like protein 1
  • MAP1A/MAP1B light chain 3 A
  • MAP1A/MAP1B LC3 A
  • Microtubule-associated protein 1 light chain 3 alpha
Background Information LC3A is composed of a heavy chain and multiple light-chain subunits. The molecular machinery required for autophagy is highly conserved in all eukaryotes as seen by the high degree of conservation of proteins involved in the formation of the autophagosome membranes. Recently, both yeast Apg8p and its rat homologue LC3-II were identified as essential constituents of autophagosome membrane as a processed form. In addition, both the yeast and human proteins exist in two modified forms produced by a series of post-translational modifications including a critical C-terminal cleavage after a conserved Gly residue, and the smaller processed form is associated with the autophagosome membranes Results revealed different regulation of the three human isoforms of LC3-II and implicate that the three isoforms may have different physiological functions. Two forms of LC3A, called LC3-I and -II, were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound.
Product Information
FormatSerum
Control
  • NIH/3T3 cell lysate
PresentationRabbit polyclonal serum containing 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-LC3 Antibody is an antibody against LC3 for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blot Analysis: A 1;1,000 dilution from a representative lot detected LC3 in 10 µg of mouse embryonic fibroblast tissue lysate and MCF-7 cell lysate.
Biological Information
ImmunogenLinear peptide corresponding to human LC3.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MAP1LC3A
Purification MethodUnpurified
UniProt Number
Molecular Weight~16 kDa observed. LC3 can occur as a doublet around ~15 kDa, indicating the presence of both LC3-I & LC3-II. An uncharacterized band may appear at ~39 and ~55 kDa in some lysates.
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: A 1;1,000 dilution of this antibody detected LC3 in 10 µg of NIH/3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Global Trade Item Number
Référence GTIN
ABC232 04053252698934