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MAB1999 Anti-Integrin α5β1 Antibody, clone HA5

MAB1999
100 µg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, PoFC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1999
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Integrin α5β1 Antibody, clone HA5
Alternate Names
  • VLA-5
Product Information
FormatPurified
PresentationPurified immunoglobulin from Protein A chromatography. Liquid in 0.02 M PB pH 7.6, 0.25 M NaCl, 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Integrin α5β1 Antibody, clone HA5 detects level of Integrin α5β1 & has been published & validated for use in FC & IP.
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
Application NotesImmunoprecipitation of VLA-5 integrins from lysate of 10E6 cell equivalent requires 2 μg of MAB1999.

Flow cytometry: 10 μg/mL

Does not detect VLA-5 by Western blot.

MAB1999 does not affect VLA-5 mediated cell adhesion to fibronectin.

Optimal working dilutions must be determined by end user.
Biological Information
CloneHA5
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with the fibronectin receptor VLA-5 (alpha-5beta-1) integrin complex.
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Pig
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Gene Symbol
  • ITGA5
  • VLA-5
  • CD49e
  • VLA5A
  • FNRA
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08648 # Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 1049 amino acids; 114536 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P08648 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µg
Global Trade Item Number
Référence GTIN
MAB1999 04053252315848