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MAB1987Z Anti-Integrin β1 Antibody, clone P4C10, azide free

MAB1987Z
100 µg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, ICC, IP, FUNC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1987Z
Replaces04-1109
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Integrin β1 Antibody, clone P4C10, azide free
Alternate Names
  • CD29
  • MAB1987
Background InformationIntegrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
References
Product Information
FormatPurified
Control
  • Human Tonsil, human skin, human lung, liver, and skeletal muscle tissues
PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-Integrin β1 Antibody, clone P4C10, azide free detects level of Integrin β1 & has been published & validated for use in ELISA, IC, IP, FUNC & WB.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Immunoprecipitation
  • Affects Function
  • Western Blotting
Application NotesInhibits attachment of UCLA-P3 cells to collagen type I (Wayner, 1991). Inhibits endodermal and keratinocytes to collagen, fibronectin and laminin. It also inhibits cell to cell adhesion. Typical dilution ranges are 1:50-1:500.

Immunoflourescence

Immunoblotting

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.
Biological Information
CloneP4C10
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with the beta-1 subunit of the integrin protein family. Appears to be human specific.Unlike many beta-1 monoclonals 4C10's epitope is not trypsin sensitive.
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryIntegrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
Gene Symbol
  • ITGB1
  • GPIIA
  • OTTHUMP00000063731
  • OTTHUMP00000046253
  • OTTHUMP00000063733
  • VLAB
  • OTTHUMP00000063732
  • MDF2
  • FNRB
  • CD29
  • MSK12
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.


SIZE: 798 amino acids; 88465 Da

SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat.

SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes.

TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).





SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
Molecular Weight88 KDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MAB1987Z 04053252616860

Documentation

Anti-Integrin β1 Antibody, clone P4C10, azide free FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-Integrin β1 Antibody, clone P4C10, azide free Certificats d'analyse

TitreNuméro de lot
Anti-Integrin 1, clone P4C10, azide free - 1940110 1940110
Anti-Integrin 1, clone P4C10, azide free - 1987049 1987049
Anti-Integrin 1, clone P4C10, azide free - 2026855 2026855
Anti-Integrin 1, clone P4C10, azide free - 2090123 2090123
Anti-Integrin 1, clone P4C10, azide free - 2325118 2325118
Anti-Integrin 1, clone P4C10, azide free - LV1757000 LV1757000
Anti-Integrin 1, clone P4C10, azide free - NG1894353 NG1894353
Anti-Integrin 1, clone P4C10, azide free - NG1914009 NG1914009
Anti-Integrin β1, clone P4C10, 2465272
Anti-Integrin β1, clone P4C10, azide free 3075610

Références bibliographiques

Aperçu de la référence bibliographiqueApplicationNº PubMed
Interplay between β1-integrin and Rho signaling regulates differential scattering and motility of pancreatic cancer cells by snail and Slug proteins.
Shields, MA; Krantz, SB; Bentrem, DJ; Dangi-Garimella, S; Munshi, HG
The Journal of biological chemistry  287  6218-29  2011

Afficher le résumé
22232555 22232555
Perisynaptic chondroitin sulfate proteoglycans restrict structural plasticity in an integrin-dependent manner.
Orlando, C; Ster, J; Gerber, U; Fawcett, JW; Raineteau, O
The Journal of neuroscience : the official journal of the Society for Neuroscience  32  18009-17, 18017a  2011

Afficher le résumé
23238717 23238717
Integrin dynamics produce a delayed stage of long-term potentiation and memory consolidation.
Babayan, AH; Kramár, EA; Barrett, RM; Jafari, M; Häettig, J; Chen, LY; Rex, CS; Lauterborn, JC; Wood, MA; Gall, CM; Lynch, G
The Journal of neuroscience : the official journal of the Society for Neuroscience  32  12854-61  2011

Afficher le résumé
22973009 22973009
Differential monocyte/macrophage interleukin-1? production due to biomaterial topography requires the ?2 integrin signaling pathway.
Angela M B Collie,Paige C S Bota,Rachel E Johns,Ronald V Maier,Patrick S Stayton
Journal of biomedical materials research. Part A  96  2010

Afficher le résumé
21105164 21105164
Spinal matrix metalloproteinase-9 contributes to physical dependence on morphine in mice.
Liu, WT; Han, Y; Liu, YP; Song, AA; Barnes, B; Song, XJ
The Journal of neuroscience : the official journal of the Society for Neuroscience  30  7613-23  2009

Afficher le résumé
20519536 20519536
Targeting of DC-SIGN on human dendritic cells by minor fimbriated Porphyromonas gingivalis strains elicits a distinct effector T cell response.
Zeituni, AE; Jotwani, R; Carrion, J; Cutler, CW
Journal of immunology (Baltimore, Md. : 1950)  183  5694-704  2009

Afficher le résumé Article en texte intégral
19828628 19828628
Integrin regulation of cytoplasmic calcium in excitatory neurons depends upon glutamate receptors and release from intracellular stores.
Lin, CY; Hilgenberg, LG; Smith, MA; Lynch, G; Gall, CM
Molecular and cellular neurosciences  37  770-80  2008

Afficher le résumé Article en texte intégral
Western Blotting18289871 18289871
Induction of cell adhesion by galectin-8 and its target molecules in Jurkat T-cells.
Hitomi Yamamoto, Nozomu Nishi, Hiroki Shoji, Aiko Itoh, Liang-Hao Lu, Mitsuomi Hirashima, Takanori Nakamura
Journal of biochemistry  143  311-24  2008

Afficher le résumé
18024965 18024965
Roles of phosphatidylinositol 3-kinase and NF-kappaB in human cytomegalovirus-mediated monocyte diapedesis and adhesion: strategy for viral persistence.
Smith, MS; Bivins-Smith, ER; Tilley, AM; Bentz, GL; Chan, G; Minard, J; Yurochko, AD
Journal of virology  81  7683-94  2007

Afficher le résumé Article en texte intégral
17507481 17507481
Integrins control dendritic spine plasticity in hippocampal neurons through NMDA receptor and Ca2+/calmodulin-dependent protein kinase II-mediated actin reorganization.
Shi, Y; Ethell, IM
The Journal of neuroscience : the official journal of the Society for Neuroscience  26  1813-22  2005

Afficher le résumé
16467530 16467530

Fiche technique

Titre
MOUSE ANTI-HUMAN INTEGRIN BETA1 MONOCLONAL ANTIBODY