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MAC140-25UG Anti-Inntag™ 10 (IT10) Antibody, clone IT10

MAC140-25UG
25 μg  
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAC140-25UG
DescriptionAnti-Inntag™ 10 (IT10) Antibody, clone IT10
Alternate Names
  • Allergen Amb t V
  • Allergen Ra5G
  • Amb t 5
Background InformationPollen allergen Amb t 5 (UniProt: P10414; also known as Allergen Amb t V, Allergen Ra5G, Amb t 5) binds to IgE and causes allergic reaction in human. Inntag ™ IT10 is a small protein domain of Ambrosia trifida (Giant ragweed) that displays strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it does not compromise cell fitness when overexpressed, and has no detectable functional and localization effects when fused to a variety of target proteins. Structure of the Inntag ™ IT10 is based on a packed beta-sheet conformation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ100
Applications
ApplicationAnti-Inntag™ 10 (IT10), clone IT10 Antibody, Cat. No. MAC140-25UG, validated for use in Western Blotting and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A representative lot detected Inntag ™ 10 (IT10) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8). Immunocytochemistry Analysis: A representative lot detected Inntag ™ 10 (IT10) in Immunocytochemistry applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).
Biological Information
ImmunogenHis-tagged recombinant peptide corresponding to 40 amino acids from Ambrosia trifida.
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone IT10-1 detects pollen allergen Abt t 5 in HEK293 cells transfected with GFP-IT10.
IsotypeIgG1
Species Reactivity
  • All
Species Reactivity NoteAmbrosia trifida.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • N/A
Purification MethodProtein G purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in transfected HEK293/GFP-IT10 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MAC140-25UG 04054839421020

Documentation