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MABF951-25UL Anti-IL-2R beta (CD122) Antibody, clone TM-beta1

Anti-IL-2R beta (CD122), clone TM-beta1, Cat. No. MABF951, is a rat monoclonal antibody that detects IL-2R beta and has been tested for use in Flow Cytometry, Function assay, Immunocytochemistry, Immunoprecipitation, Neutralizing, and Western Blotting.

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Research Category: Inflammation & Immunology Gene Symbol: Il2rb UniProt Number: P16297
MABF951-25UL
25 µL  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFC, Function Assay, ICC, IP, NEUT, WBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF951-25UL
DescriptionAnti-IL-2R beta (CD122) Antibody, clone TM-beta1
Alternate Names
  • Interleukin-2 receptor subunit beta
  • IL-2 receptor subunit beta
  • IL-2R subunit beta
  • High affinity IL-2 receptor subunit beta
  • p70-75
  • CD122
Background InformationInterleukin-2 receptor subunit beta (UniProt: P16297; also known as IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, High affinity IL-2 receptor subunit beta, p70-75, CD122) is encoded by the Il2rb gene (Gene ID: 16185) in murine species. IL-2R is a non-covalent dimer of an alpha and a beta subunit. It can exist as a high affinity dimer, an intermediate affinity monomer of beta subunit, and as a low affinity monomer of alpha subunit. IL-2R beta is a single-pass type I membrane protein. The beta subunit is involved in receptor mediated endocytosis and it transduces the mitogenic signals of IL-2. IL-2R beta is synthesized with a signal peptide (aa 1-26), which is cleaved off to produce the mature form that has an extracellular domain (aa 27-240), a transmembrane domain (aa 241-268), and a long cytoplasmic domain (aa 269-539). IL-2R beta contains a WSXWS motif (aa 221-225), which is essential for its proper folding and thereby efficient intracellular transport. It also has a Box 1 motif (aa 281-289) that is required for JAK interaction and/or activation.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2b in PBS without azide.
Quality LevelMQ100
Applications
ApplicationAnti-IL-2R beta (CD122), clone TM-beta1, Cat. No. MABF951, is a rat monoclonal antibody that detects IL-2R beta and has been tested for use in Flow Cytometry, Function assay, Immunocytochemistry, Immunoprecipitation, Neutralizing, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Function Assay
  • Immunocytochemistry
  • Immunoprecipitation
  • Neutralizing
  • Western Blotting
Application NotesNeutralizing Analysis: Freshly isolated mouse splenocytes pretreated with different amounts of MABF951, Anti-IL-2R beta (CD122), clone TM- 1 showed a co-relation of the amount of antibody used vs percent of cells stained with FITC-labeled antibody. In antibody titration, as the concentration of unlabeled anti-IL-2R beta (CD122) is decreased less inhibition is observed as a result.

Inhibits Activity/Function: A representative lot abolished the binding of IL2 ligand to IL-2R beta (CD122). (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8; Yokoyama, S., et. al. (2009). Proc Natl Acad Sci USA. 106(37):15849-54).

Flow Cytometry Analysis: A representative lot detected IL-2R beta (CD122) in Flow Cytometry applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).

Western Blotting Analysis: A representative lot detected IL-2R beta (CD122) in Western Blotting applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).

Immunoprecipitation Analysis: A representative lot immunoprecipitated IL-2R beta (CD122) in Immunoprecipitation applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).

Immunocytochemistry Analysis: A representative lot detected IL-2R beta (CD122) in Immunocytochemistry applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).
Biological Information
ImmunogenTART-mB whole cells
CloneTM-beta 1
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone TM-beta 1 is a rat monoclonal antibody that detects IL-2R beta subunit in murine species.
IsotypeIgG2bκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Il2rb
Purification MethodProtein G purified
UniProt Number
Molecular Weight60.54 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABF951-25UL 04054839441257