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MABE71 Anti-Histone Antibody, Pan, clone F152.C25.WJJ

MABE71
100 µg  
Purchase on Sigma-Aldrich

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Species ReactivityKey ApplicationsHostFormatAntibody Type
H, BWB, ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE71
ReplacesMAB052
DescriptionAnti-Histone Antibody, Pan, clone F152.C25.WJJ
Alternate Names
  • Histone H1.4
  • Histone H1b
Background InformationThis antibody detects histone H1 and nuclesomal core proteins histones H2a, H2b, H3, H4. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Histone H1 is a nuclear protein necessary for condensation of nucleosome chains into higher order structures.
Product Information
FormatPurified
Control
  • Jurkat cell lysate
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Histone Antibody, Pan, clone F152.C25.WJJ is a Mouse Monoclonal Antibody for detection of Histone also known as Histone H1.4, Histone H1b & has been validated in WB, ELISA.
Key Applications
  • Western Blotting
  • ELISA
Application NotesELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Mizoguchi, E., et al. (1997). Gastroenterology. 113:1828-1835.)
Biological Information
CloneF152C25W
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Bovine
Antibody TypeMonoclonal Antibody
Gene Symbol
  • HIST1H1E
  • H1F4
Purification MethodProtein G
UniProt Number
Molecular Weight ~14-33 kDa observed
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Histone H1 and core proteins on 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Référence GTIN
MABE71 04053252416682