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MABS1233 Anti-HMG-CoA Reductase Antibody, clone IgG-A9

Detect HMG CoA Reductase using this mouse monoclonal Anti-HMG-CoA Reductase, clone IgG-A9, Cat. No. MABS1233, validated for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

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Research Category: Signaling Gene Symbol: HMGCR UniProt Number: P00347
MABS1233
100 µL  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Ht, RICC, WB, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1233
DescriptionAnti-HMG-CoA Reductase Antibody, clone IgG-A9
Alternate Names
  • 3-hydroxy-3-methylglutaryl-coenzyme A reductase
  • HMG-CoA reductase
Background Information3-hydroxy-3-methylglutaryl-coenzyme A reductase (EC 1.1.1.34; UniProt P00347; also known as HMG-CoA reductase) is encoded by the HMGCR gene (Gene ID 100756363) in Cricetulus griseus (Chinese hamster). HMG-CoA reductase catalyzes the conversion of HMG-CoA to mevalonic acid, the rate-limiting enzyme of the mevalonate-isoprenoid biosynthesis (MIB) pathway responsible for the production of cholesterol and other isoprenoids, as well as farnesyl pyrophosphate (FPP) required for protein prenylation. HMG-CoA reductase is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor as well as oxidized cholesterol species. Competitive inhibitors against HMG-CoA reductase induce LDL receptors expression in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol. HMG-CoA reductase is a multitransmembrane ER protein with an N-terminal sterol-sensing domain (SSD; a.a. 61-218) and a C-terminal catalytic domain (a.a. 450-888) whose activity is inhibited upon SSD binding by cholesterol. Numerous studies have implicated the involvement of HMG-CoA reductase and mevalonate pathway in carcinogenesis.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect HMG CoA Reductase using this mouse monoclonal Anti-HMG-CoA Reductase, clone IgG-A9, Cat. No. MABS1233, validated for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: A 1:100 dilution from a representative lot detected in 15 µg of membrane extract from CHO7 cells treated overnight with 0.01 mM compactin (Courtesy of Linda Donnelly, Department of Molecular Genetics, UT Southwestern Medical Center, Dallas, TX, USA).

Immunocytochemistry Analysis: A representative lot detected HMG CoA reductase-positive ER membrane structures by indirect immunofluorescence staining of paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized CHO-K1 cells cultured with compactin, mevalonate, and MG-132, in the presence or absence of 25-hydroxycholesterol (25-HC) (Hartman, I.Z., et al. (2010). J. Biol. Chem. 285(25):19288-19298).

Immunoprecipitation Analysis: A representative lot immunodepleted HMC-CoA reductase activity from compactin-adapted CHO cell (UT-1) extract (Liscum, L., et al. (1983). J. Biol. Chem. 258(13) 8450-8455).

Western Blotting Analysis: A representative lot detected 25-hydroxycholesterol (25-HC) treatment-induced HMC-CoA reductase dislocation from ER membrane to the cytosol and lipid droplet in CHO-K1 cells cultured with lipoprotein-deficient serum in the presence of compactin, MG-132, with or without mevalonate. Cellular VCP knockdown prevented sterol-induced HMC-CoA reductase cytosolic, but not lipid droplet, translocation (Hartman, I.Z., et al. (2010). J. Biol. Chem. 285(25):19288-19298).

Western Blotting Analysis: A representative lot detected 25-hydroxycholesterol (25-HC) treatment-induced ubiquitination of HMC-CoA reductase in SV-589 human fibroblasts cultured with compactin and mevalonate in the presence of proteasome inhibitor MG-132. Cellular knockdown of gp78A, but not gp78B or Hrd1, reduced 25-HC-induced HMC-CoA reductase ubiquitination (Song, B.L., et al. (2005). Mol. Cell. 19(6):829-840).

Western Blotting Analysis: A representative lot detected 25-hydroxycholesterol (25-HC) treatment-induced degradation of HMC-CoA reductase in SV-589 human fibroblasts cultured with compactin and mevalonate. Cellular knockdown of gp78A, VCP, or Insig-1/-2 suppressed sterol-induced HMC-CoA reductase degradation (Song, B.L., et al. (2005). Mol. Cell. 19(6):829-840).

Western Blotting Analysis: A representative lot detected HMC-CoA reductase in both membrane and nuclear fractions from HEK-293S cells cultured with compactin and mevalonate. Additional treatment with 25-hydroxycholesterol (25-HC) resulted in HMC-CoA reductase degradation (Sever, N., et al. (2003). Mol. Cell. 11(1):25-33).

Western Blotting Analysis: A representative lot detected an upregulated HMC-CoA reductase expression in compactin-adapted CHO cells (UT-1), as well as a loss of HMC-CoA reductase expression in UT-1 cells cultured in the presence of LDL (Liscum, L., et al. (1983). J. Biol. Chem. 258(13) 8450-8455).

Western Blotting Analysis: A representative lot detected a greater HMC-CoA reductase upregulation in liver microsome preparation from rats on diet supplemented with cholestyramine and mevinolin than with cholestyramine alone, while supplementation with cholesterol in addition to cholestyramine and mevinolin downregulated liver HMC-CoA reductase (Liscum, L., et al. (1983). J. Biol. Chem. 258(13) 8450-8455).
Biological Information
ImmunogenMembrane preapration from compactin-adapted CHO cells (Liscum, L., et al. (1983). J. Biol. Chem. 258(13) 8450-8455).
CloneIgG-A9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone IgG-A9 targets an epitope within HMC-CoA reductase catalytic domain (a.a. 450 887).
IsotypeIgG1κ
Species Reactivity
  • Human
  • Hamster
  • Rat
Species Reactivity NoteHamster, Human, Rat.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • HMGCR
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~92 kDa observed. 97.08 kDa (hamster), 97.48/92.02/99.75 kDa (human isoform 1/2/3 (HMGCR-1b)), 96.69 kDa (rat) calculated.. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIdentity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABS1233 04054839081361