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MABD90 Anti-HLA-DRB1 Antibody, clone DJ9

MABD90
100 µL  
Purchase on Sigma-Aldrich

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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, IHC, FCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABD90
DescriptionAnti-HLA-DRB1 Antibody, clone DJ9
Alternate Names
  • Major histocompatibility complex, class II
  • DRB1
  • DRB1
Background InformationHLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. HLA-DRB1 binds peptides derived from antigens that access the endocytic route of antigen presenting cells and presents them on the cell surface for recognition by the CD4 T-cells. Class II molecules are expressed in antigen presenting cells B lymphocytes, dendritic cells, macrophages. HLA-Drb1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. HLA-DRB1, clone DJ9, has been shown to be a relevant marker of bone marrow derived mesenchymal stem cells and their osteogenic derivatives (Andersen, D., et al., 2011).
References
Product Information
FormatPurified
Control
  • Daudi cell lysates
PresentationPurified mouse monoclonal IgG2aλ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect HLA-DRB1 using this Anti-HLA-DRB1 Antibody, clone DJ9 validated for use in Western Blotting, ICC, IHC, Flow Cytometry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
  • Flow Cytometry
Application NotesThis antibody has been shown to work in Immunocytochemistry (Andersen, D., et al. (2011)). This antibody has been shown to work in Immunohistochemistry (Andersen, D., et al. (2011)). This antibody has been shown to work in Flow Cytometry (Andersen, D., et al. (2011)).
Biological Information
ImmunogenWhole hMSC-TERT Cells
CloneDJ9
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes human HLA-DRB1.
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HLA-DRB1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~30 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in Daudi cell lysates. Western Blot Analysis: A 1:100,000 dilution of this antibody detected HLA-DRB1 in Duadi cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABD90 04053252698835

Documentation

Anti-HLA-DRB1 Antibody, clone DJ9 FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-HLA-DRB1 Antibody, clone DJ9 Certificats d'analyse

TitreNuméro de lot
Anti-HLA-DRB1, clone DJ9 2476792
Anti-HLA-DRB1, clone DJ9 - 2536491 2536491
Anti-HLA-DRB1, clone DJ9 - 3470767 3470767
Anti-HLA-DRB1, clone DJ9 - 3609385 3609385
Anti-HLA-DRB1, clone DJ9 - 3800850 3800850
Anti-HLA-DRB1, clone DJ9 - 3888816 3888816
Anti-HLA-DRB1, clone DJ9 - 4163449 4163449
Anti-HLA-DRB1, clone DJ9 - 4204608 4204608
Anti-HLA-DRB1, clone DJ9 - Q2060585 Q2060585
Anti-HLA-DRB1, clone DJ9 -2550996 2550996