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MAB397A4 Anti-GluR2 Antibody, clone 6C4, Alexa Fluor® 488 Conjugate

This Anti-GluR2 Antibody, clone 6C4, Alexa Fluor 488 Conjugate is validated for use in IH, IC for the detection of GluR2.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: Gria2, GLUR2, GLURB, GluR-2, GluR-B UniProt Number: P19491
MAB397A4
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RIHC, ICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberMAB397A4
DescriptionAnti-GluR2 Antibody, clone 6C4, Alexa Fluor® 488 Conjugate
Alternate Names
  • Glutamate receptor 2
  • GluR-2
  • AMPA-selective glutamate receptor 2
  • GluR-B
  • GluR-K2
  • Glutamate receptor ionotropic, AMPA 2
Background InformationGlutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.
References
Product Information
FormatAlexaFluor®488
Control
  • Adult mouse brain tissue
PresentationPurified mouse monoclonal IgG2a conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationThis Anti-GluR2 Antibody, clone 6C4, Alexa Fluor 488 Conjugate is validated for use in IH, IC for the detection of GluR2.
Key Applications
  • Immunohistochemistry
  • Immunocytochemistry
Application NotesImmunocytochemistry analysis: A 1:100 dilution from a representative lot detected GluR2 in rat E18 primary cortex cells.
Biological Information
ImmunogenRecombinant fusion protein TrpE-GluR2
EpitopeExtracellular domain
Clone6C4
HostMouse
SpecificityRecognizes the large N-terminal extracellular domain of Glutamate Receptor 2 (GluR2). No cross-reactivity observed with other AMPA/Kainate GluR subunits.
IsotypeIgG2a
Species Reactivity
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryGlutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene.
Gene Symbol
  • Gria2
  • GLUR2
  • GLURB
  • GluR-2
  • GluR-B
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

SIZE: 883 amino acids; 98,688 Da

SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4 (By similarity). Interacts with CACNG2, PRKCABP, GRIP1 and GRIP2. Interacts with NSF via its C-terminus. Part of a complex containing GRIA2, NSF and NAPA and/or NAPB.

SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note= Interaction with CACNG2 promotes cell surface expression.

TISSUE SPECIFICITY: Detected in forebrain. Detected in dendrites of neuronal cells.

SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
Molecular WeightThe unconjugated parent antibody (Cat. No. MAB397) has an observed molecular weight at ~102 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in adult mouse brain tissue.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected GluR2 in adult mouse brain tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MAB397A4 04053252420870

Documentation

Anti-GluR2 Antibody, clone 6C4, Alexa Fluor® 488 Conjugate FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-GluR2 Antibody, clone 6C4, Alexa Fluor® 488 Conjugate Certificats d'analyse

TitreNuméro de lot
Anti-GluR2, clone 6C4, Alexa Fluor#174; 488 Conjugate - 2029164 2029164
Anti-GluR2, clone 6C4, Alexa Fluor#174; 488 Conjugate - 2161206 2161206
Anti-GluR2, clone 6C4, Alexa Fluor#174; 488 Conjugate - 2266773 2266773
Anti-GluR2, clone 6C4, Alexa Fluor#174; 488 Conjugate - NRG1922537 NRG1922537
Anti-GluR2, clone 6C4, Alexa Fluor® -2760517 2760517
Anti-GluR2, clone 6C4, Alexa Fluor® 488 Conjugate 2956761
Anti-GluR2, clone 6C4, Alexa Fluor® 488 Conjugate 2794938
Anti-GluR2, clone 6C4, Alexa Fluor® 488 Conjugate - 2377087 2377087
Anti-GluR2, clone 6C4, Alexa Fluor® 488 Conjugate - 2315733 2315733
Anti-GluR2, clone 6C4, Alexa Fluor® 488 Conjugate - 2361550 2361550
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Brochure

Titre
Pathways and Biomarkers of Glutamatergic Synapse Flyer