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05-1403 Anti-Giα2 Antibody, clone L5

This Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2.

Anti-Giα2 Antibody, clone L5 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Signaling Research Sub-Category: GPCR, cAMP/cGMP & Calcium Signaling Gene Symbol: GNAI2, GNAI2B, WUGSC:H_LUCA15.1, H_LUCA15.1, H_LUCA16.1, GIP, WUGSC:H_LUCA16.1 UniProt Number: P04899
05-1403
100 µg  
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Replacement Information
Replacement Information05-14013 is a recommended replacement for MAB3077

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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, B, M, H, GpWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue Number05-1403
ReplacesMAB3077
DescriptionAnti-Giα2 Antibody, clone L5
References
Product Information
FormatPurified
Control
  • Huvec cell lysate
PresentationPurified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesOptimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer

Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes
Biological Information
ImmunogenRecombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243).
CloneL5
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes Giα-2.
IsotypeIgG2b
Species Reactivity
  • Rat
  • Bovine
  • Mouse
  • Human
  • Guinea Pig
Species Reactivity NoteStrong reactivity with bovine (100%), mouse (69%), rat (69%), human (62%) and guinea pig (43%). Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GNAI2
  • GNAI2B
  • WUGSC:H_LUCA15.1
  • H_LUCA15.1
  • H_LUCA16.1
  • GIP
  • WUGSC:H_LUCA16.1
Purification MethodPurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P04899 # Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli.
SIZE: 355 amino acids; 40451 Da
SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1 (By similarity). Interacts with UNC5B.
SIMILARITY: SwissProt: P04899 ## Belongs to the G-alpha family. G(i/o/t/z) subfamily.
Molecular WeightLabels a single protein band of 39-42 kDa in bovine and rat membranes.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on Huvec lysates.

Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C for 1 year from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
05-1403 04053252324727

Documentation

Anti-Giα2 Antibody, clone L5 FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-Giα2 Antibody, clone L5 Certificats d'analyse

TitreNuméro de lot
Anti-Gi2, clone L5 - NG1578610 NG1578610
Anti-Gi2, clone L5 - NG1726943 NG1726943
Anti-Gi2, clone L5 - NG1811040 NG1811040
Anti-Gia2, clone L5 - JH1769942 JH1769942
Anti-Giα2, clone L5 - 2395684 2395684
Anti-Giα2, clone L5 - 2437347 2437347
Anti-Giα2, clone L5 - 3248388 3248388
Anti-Giα2, clone L5 - 3980280 3980280
Anti-Giα2, clone L5 -2658751 2658751
Anti-Giα2, clone L5 -2887673 2887673
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