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MABC1127-25UL Anti-FGFR-4 Antibody, clone 29.2

Anti-FGFR-4, clone 29.2, Cat. No. MABC1127, is a rabbit monoclonal antibody that detects Fibroblast growth factor receptor 4 and has been tested for use in Flow Cytometry.

Anti-FGFR-4 Antibody, clone 29.2 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Signaling Gene Symbol: FGFR4 UniProt Number: P22455
MABC1127-25UL
25 µL  
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Replacement Information

Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCRbCulture SupernatantMonoclonal Antibody
Description
Catalogue NumberMABC1127-25UL
DescriptionAnti-FGFR-4 Antibody, clone 29.2
Alternate Names
  • Fibroblast growth factor receptor 4
  • EC: 2.7.10.1
  • CD334
Background InformationFibroblast growth factor receptor 4 (UniProt: P22455; also known as EC: 2.7.10.1, FGFR-4, CD334) is encoded by the FGRF4 (also known as JTK2, TKF) gene (Gene ID: 2264) in human. FGFR-4 is a highly conserved, single-pass type I membrane protein with tyrosine kinase activity that serves as a cell surface receptor for fibroblast growth factors and regulates cell proliferation differentiation, and migration. FGFR-4 is expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines. In the absence of a ligand, it is present as a monomer in an inactive conformation. Upon ligand binding it homodimerizes into an active conformation with autophosphorylation of tyrosine residues. FGFR-4 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. It contains an extracellular domain (aa 22-369), a transmembrane domain (aa 370-390), and a cytoplasmic domain (aa 391-802). FGFR-4 undergoes N-glycosylation, and when not fully glycosylated it can undergo proteasomal degradation. FGFR-4 signaling is down-regulated by receptor internalization and degradation. MMP14 is reported to promote internalization and degradation of FGFR-4. Mutations that lead to constitutive kinase activation or impair normal FGFR-4 inactivation lead to aberrant signaling leading to tumor growth. (Ref.: Turkington, RC et al. (2014). Cell Death Dis. 5; e1046).
References
Product Information
FormatCulture Supernatant
PresentationRabbit monoclonal antibody in supernatant without preservatives.
Applications
ApplicationAnti-FGFR-4, clone 29.2, Cat. No. MABC1127, is a rabbit monoclonal antibody that detects Fibroblast growth factor receptor 4 and has been tested for use in Flow Cytometry.
Key Applications
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: A 1:4 dilution from a representative lot detected FGFR-4 in one million LNCaP cells.
Biological Information
ImmunogenHis-tagged recombinant extracellular domain of human fibroblast growth factor receptor 4 (FGFR-4).
Epitopeextracellular domain
Clone29.2
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit monoclonal antibody detects human fibroblast growth factor receptor 4 (FGFR-4). It targets an epitope with in the extracellular domain.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FGFR4
Purification MethodUnpurified
UniProt Number
Molecular Weight87.95 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in DU-145 cells.

Flow Cytometry Analysis: A 1:4 dilution of this antibody detected FGFR-4 in one million DU-145 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABC1127-25UL 04054839550904