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ABC501 Sigma-AldrichAnti-EDG3 Antibody

Anti-EDG3 Antibody is an antibody against EDG3 for use in Western Blotting and Immunohistochemistry.

Anti-EDG3 Antibody : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

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Références bibliographiques
Brochures
Informations techniques

Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: S1PR3, EDG3 UniProt Number: Q99500

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Tableau de caractéristiques principal

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IHC	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABC501
Description	Anti-EDG3 Antibody
Alternate Names	EDG3 Sphingosine 1-phosphate receptor 3 S1P receptor 3 S1P3 Endothelial differentiation G-protein coupled receptor 3 Sphingosine 1-phosphate receptor Edg-3 S1P receptor Edg-3
Background Information	EDG3, also known as Sphingosine 1-phosphate receptor 3, S1P receptor 3 or S1P3 or Endothelial differentiation G-protein coupled receptor 3 or Sphingosine 1-phosphate receptor Edg-3 or S1P receptor Edg-3, and encoded by the gene name S1PR3 or EDG3, EDG3 is a member of the EDG family of receptors, which are G protein-coupled receptors. It has been identified as a functional receptor for sphingosine 1-phosphate (S1P) and likely contributes to the regulation of angiogenesis and vascular endothelial cell function.

References

Product Information
Format	Affinity Purified
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ300

Applications
Application	Anti-EDG3 Antibody is an antibody against EDG3 for use in Western Blotting and Immunohistochemistry.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected EDG3 in human kidney and human liver tissue.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to the C-terminus of human EDG3.
Epitope	C-terminus
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Species Reactivity	Human
Species Reactivity Note	Human. Predicted to react with Feline based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_005217
Gene Symbol	S1PR3 EDG3
Purification Method	Affinity Purfied
UniProt Number	Q99500
Molecular Weight	~52 kDa observed. The calculated molecular weight of this protein is 42 kDa, but can be observed at ~52 kDa due to high glycosylation (Magrassi, L., et al. (2010). Cancer Letters. 290(1):36-42). Uncharacterized band(s) may appear in some lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in Caco-2 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected EDG3 in 10 µg of Caco-2 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Référence	GTIN
ABC501	04055977151725

Documentation

Anti-EDG3 Antibody Certificats d'analyse

Titre	Numéro de lot
Anti-EDG3 - 3397944	3397944
Anti-EDG3 -Q2559136	Q2559136

Références bibliographiques

Aperçu de la référence bibliographique	Nº PubMed
Cancer letters Magrassi, L; Marziliano, N; Inzani, F; Cassini, P; Chiaranda, I; Skrap, M; Pizzolito, S; Arienta, C; Arbustini, E Cancer letters 290 36-42 2009 Afficher le résumé By qPCR we found that EDG3 and SHC3 were amplified in 60% of ependymomas but none in choroid plexus papillomas. In ependymomas EDG3 and SHC3 amplification increased Shc3 protein levels while EDG3 was less affected. Both proteins were co-immunoprecipitated from ependymoma and Shc3 was tyrosine phosphorylated thus presumably active. We showed by digestion with N-glycosidase-F that EDG3 was glycosylated indicating that EDG3 protein was not retained in the endoplasmic reticulum. The co-immunoprecipitation of Shc3 and EDG3 proteins from ependymomas with amplification of SHC3 and EDG3 genes suggests that the two proteins co-operate and are important for ependymomas in vivo.	19748727

Aperçu de la référence bibliographique

Nº PubMed

Brochure

Titre
NPI Flyer- Epigenetics and Nuclear Function Feature

Informations techniques

Titre
White Paper: Further considerations of antibody validation and usage.

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Catégories

Life Science Research > Antibodies and Assays > Primary Antibodies

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