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MABT118 Anti-Desmoglein-1 Antibody, clone 32-2B

MABT118
100 µg  
Purchase on Sigma-Aldrich

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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IF, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT118
DescriptionAnti-Desmoglein-1 Antibody, clone 32-2B
Alternate Names
  • Desmoglein-1
  • Desmosomal glycoprotein 1
  • DG1
  • DGI
  • Pemphigus foliaceus antigen
Background InformationDesmoglein-1 (DSG1) is a transmembrane glycoprotein belonging to the family of cadherins. It is one of the main structural components of desmosomal junctions which are abundant in tissues undergoing frequent mechanical stress, such as the heart, epidermis, lymphatic endothelial cells, and meninges. The desmoglein-1 subtype is highly expressed in the epidermis. Like other cadherins, desmoglein-1 contains multiple calcium binding sites and a cell-adhesion recognition site which facilitates intercellular adhesions. Desmoglein-1 is regulated by calcium and PKCα signaling, and is also coupled to intermediate filaments of the cytoskeleton. Desmosomes play a critical role in cell and tissue stability, and may contribute to the progression of cancer.
Product Information
FormatPurified
Control
  • Human tonsil tissue lysate.
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Desmoglein-1, clone 32-2B detects levels of Desmoglein-1 proteins & has been published & validated for use in WB, IF, IHC & ICC.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunocytochemistry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Desmoglein-1 in human tonsil tissues.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Desmoglein-1 in human tonsil tissues.

Western Blot Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in bovine epidermal desmosomal cores and in human keratinocyte cell lysates. (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.).

Immunofluorescent Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in MDCK cells (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in human epidermal tissue (Maruani, A., et al. (2008). Am J Clin Pathol. 130(3):369-374.).
Biological Information
ImmunogenBovine Desmoglein-1 from nasal epidermis
Clone32-2B
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Bovine based on immunogen design.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DSG1
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein by similarity. Cell junction > desmosome.

TISSUE SPECIFICTY: Epidermis, muzzle, tongue and esophagus.

DOMAIN: Calcium may be bound by the cadherin-like repeats.

SEQUENCE SIMILARITIES: Contains 4 cadherin domains.
Molecular Weight~150 kDa observed. This protein may be glycosolated. Uniprot describes a molecular weight at ~112 kDa; however, Desmoglein-1 has been observed at ~150 kDa (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.).
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human tonsil tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Desmoglein-1 in 10 µg of human tonsil tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Référence GTIN
MABT118 04053252644474