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ST1672 Anti-DPYD Mouse mAb (7D4)

Anti-DPYD, mouse monoclonal, clone 7D4, recognizes the ~100 kDa DPYD protein in HeLa and HeLa NE cells. It is validated for use in ELISA, Western blotting, and immunocytochemistry.

Anti-DPYD Mouse mAb (7D4) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Dihydropyrimidine Dehydrogenase

ST1672
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Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

RéférenceConditionnement Qté
ST1672-100UG 100 μg
Description
OverviewRecognizes the ~100 kDa DPYD protein in HeLa and HeLa NE cells.
Catalogue NumberST1672
Brand Family Calbiochem®
SynonymsAnti-Dihydropyrimidine Dehydrogenase
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells, HeLa NE cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 1-111 human DPYD, expressed as a GST fusion protein
Clone7D4
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
ST1672-100UG 04055977223835

Documentation

Anti-DPYD Mouse mAb (7D4) FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-November-2010 RFH
SynonymsAnti-Dihydropyrimidine Dehydrogenase
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
DescriptionPurified mouse monoclonal antibody. Recognizes the ~100 kDa DPYD protein.
BackgroundThe protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 1-111 human DPYD, expressed as a GST fusion protein
Clone7D4
IsotypeIgG₁
Specieshuman
Positive controlHeLa cells, HeLa NE cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review