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ABN40 Anti-DLK1 Antibody

This Anti-DLK1 Antibody is validated for use in WB, IH for the detection of DLK1.

Anti-DLK1 Antibody : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: DLK1, DLK UniProt Number: P80370
ABN40
100 µg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN40
DescriptionAnti-DLK1 Antibody
Alternate Names
  • Protein delta homolog 1
  • DLK-1
  • pG2
  • Fetal antigen 1
  • FA1
Background InformationDelta-like 1 homolog (DLK1), also known as fetal antigen 1 (FA1) and preadipocyte factor 1 (pref-1), is a transmembrane protein containing 6 epidermal growth factor repeats whose expression is known to modulate differentiation signals. Studies have shown DLK1 is involved in differentiation of several cell types, including adipocytes. Studies have shown deficiencies in DLK1 displaying growth retardation, obesity, skeletal malformation, and increased serum lipid. DLK1 is a paternally expressed imprinted gene that plays a role in normal development and in the maintence of homeostatsis of adipose tissue mass.
References
Product Information
FormatAffinity Purified
Control
  • HepG2 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-DLK1 Antibody is validated for use in WB, IH for the detection of DLK1.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected DLK1 in mouse embryonic tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human DLK1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. [provided by RefSeq, Mar 2010].
Gene Symbol
  • DLK1
  • DLK
Purification MethodAffinity Purfied
UniProt Number
UniProt SummarySUBUNIT STRUCTURE: Monomer.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

TISSUE SPECIFICITY: Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.

PTM: N- and O-glycosylated.

SEQUENCE SIMILARITIES: Contains 6 EGF-like domains.

SEQUENCE CAUTION: The sequence CAA35582.1 differs from that shown. Reason: Frameshift at positions 22, 108 and 199.
Molecular Weight~47 kDa observed. The calculated molecular weight of this protein is 41 kDa, but can be observed at ~41-55 kDa due to high glycosylation.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected DLK1 on 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
ABN40 04053252536595