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ABN458 Anti-Choline Transporter Antibody

Anti-Choline Transporter, Cat. No. ABN458, is a rabbit polyclonal antibody that detects Choline Transporter and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Choline Transporter Antibody : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: SLC5A7, CHT1 UniProt Number: Q9GZV3
ABN458
100 µg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN458
ReplacesAB5960;AB5966
DescriptionAnti-Choline Transporter Antibody
Alternate Names
  • High affinity choline transporter 1
  • Hemicholinium-3-sensitive choline transporter
  • CHT
  • Solute carrier family 5 member 7
Background InformationHigh affinity choline transporter 1 (UniProt: Q9GZV3; also known as Hemicholinium-3-sensitive choline transporter, CHT, Solute carrier family 5 member 7) is encoded by the SLC5A7 (also known as CHT1) gene (Gene ID: 60482) in human. CHT is a multi-pass membrane protein with six extracellular domains, eleven transmembrane domains, and six cytoplasmic domains. It is expressed in cholinergic neurons in putamen, spinal cord, and medulla and serves as a transmembrane transporter that imports choline from the extracellular space into neurons with high affinity in a sodium and chloride ion-dependent manner. In neurons it is acetylated by the action of choline acetyltransferase to generate acetylcholine. Choline uptake is shown to be the rate-limiting step in acetylcholine synthesis. The majority of CHT is localized intracellularly including a proportion present on acetylcholine-containing synaptic vesicles. Diminished levels of acetylcholine in selected areas of the nervous system are linked to several neurodegenerative diseases. Mutation in SLC5A7 gene have been linked to neuronopathy and myasthenic syndrome that is characterized by failure of neuromuscular transmission, fatigability, and muscle atrophy and weakness. (Ref.: Bauche, S., et al. (2016). Am. J. Hum. Genet. 99(3); 753-761; Barwick, KES., et al. (2012). Am. J. Hum. Genet. 91(6); 1103-1107).
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-Choline Transporter, Cat. No. ABN458, is a rabbit polyclonal antibody that detects Choline Transporter and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesTested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Choline Transporter in rat spinal cord and human cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 77 amino acids from the C-terminal region of human High affinity choline transporter.
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects high affinity choline transporter. It targets an epitope within the C-terminal region.
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with Human, Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SLC5A7
  • CHT1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~63 kDa observed; 63.2 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat spinal cord tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Choline Transporter in Rat spinal cord tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
ABN458 04053252941436

Documentation

Anti-Choline Transporter Antibody FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-Choline Transporter Antibody Certificats d'analyse

TitreNuméro de lot
Anti-Choline Transporter - Q2343010 Q2343010
Anti-Choline Transporter - 3218873 3218873
Anti-Choline Transporter - 3288744 3288744
Anti-Choline Transporter - 3474173 3474173
Anti-Choline Transporter - 3745922 3745922
Anti-Choline Transporter - 3783265 3783265
Anti-Choline Transporter - 3857741 3857741
Anti-Choline Transporter - 3861356 3861356
Anti-Choline Transporter - 3956639 3956639
Anti-Choline Transporter - 4050752 4050752
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