Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

PC205L Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb

Anti-CGRP, rabbit polyclonal, recognizes the α-CGRP in rat amygdala and spinal cord. It is validated for immunofluorescence, & for use with frozen, paraffin, and free-floating sections.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-CGRP

PC205L
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
M, RRbPolyclonal Antibody

Products

RéférenceConditionnement Qté
PC205L-100UL Ampoule plast. 100 ul
Description
OverviewRecognizes the α-calcitonin gene related peptide in rat amygdala and spinal cord.
Catalogue NumberPC205L
Brand Family Calbiochem®
SynonymsAnti-CGRP
Application Data
Detection of rat calcitonin gene related peptide by immunohistochemistry. Sample: Rat amygdala and spinal cord. Primary antibody: Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb (Cat. No. PC205L) (1:400). Detection: fluorescence.
References
ReferencesSudhof, T.C. 1995. Nature 375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlRat amygdala or spinal cord
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesParaffin Sections, Free Floating Sections, Immunofluorescence Maatkamp, A., et al. 2004. Eur. J. Neurosci. 20, 14.
Key Applications Free-floating Sections
Frozen Sections
Immunofluorescence
Paraffin Sections
Application NotesFree Floating Sections (see application references)
Frozen Sections (1:2000-1:4000, ABC detection)
Immunofluorescence (1:100-1:200, see application references)
Paraffin Sections (see application references; heat pre-treatment required; see comments)
Application CommentsAntibody specificity was examined in the rat amygdala and spinal cord. Staining is completely eliminated by pre-treatment of antibody with 10-5M rat α-CGRP and partially eliminated by preabsorption with rat β-CGRP. Preabsorption with calcitonin resulted in no loss of immunostaining. For staining paraffin sections, heat sections to 80°C in 40 mM sodium citrate buffer, pH 8.5. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrat α-calcitonin gene related peptide (α-CGRP)
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Carefully reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
PC205L-100UL 04055977209532

Documentation

Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb Certificats d'analyse

TitreNuméro de lot
PC205L

Références bibliographiques

Aperçu de la référence bibliographique
Sudhof, T.C. 1995. Nature 375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 RFH
SynonymsAnti-CGRP
ApplicationFree Floating Sections (see application references)
Frozen Sections (1:2000-1:4000, ABC detection)
Immunofluorescence (1:100-1:200, see application references)
Paraffin Sections (see application references; heat pre-treatment required; see comments)
Application Data
Detection of rat calcitonin gene related peptide by immunohistochemistry. Sample: Rat amygdala and spinal cord. Primary antibody: Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb (Cat. No. PC205L) (1:400). Detection: fluorescence.
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the α-CGRP protein.
BackgroundNeurotransmitters can be defined as chemical substances that are released by the presynaptic neuron before being bound by receptors on the postsynaptic cell surface. It has been well established that the nervous system utilizes neurotransmitters for cell signaling. Neurotransmitters can be divided into two groups; low molecular weight transmitter molecules such as biogenic amines, purines, or amino acids, and bioactive peptides, of which more than 50 members have been identified in the mammalian central nervous system. Many low molecular weight neurotransmitters are produced within the axon terminal from common cellular metabolites and packaged into synaptic vesicles. In contrast, neuropeptides are synthesized in the cell body and packaged into large dense core vesicles for delivery to the presynaptic membrane. The transport and delivery of vesicles containing neurotransmitter is a highly regulated and complex process. The calcitonin gene peptide superfamily consists of calcitonin, calcitonin gene-related peptide (CGRP), amylin, and adrenomedullin. CGRP is a 37 amino acid neuropeptide that shows widespread distribution and acts as a potent vasodilator. The immunolocalization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
HostRabbit
Immunogen speciesRat
Immunogenrat α-calcitonin gene related peptide (α-CGRP)
IsotypeIgG
Speciesmouse, rat
Positive controlRat amygdala or spinal cord
FormLyophilized
FormulationUndiluted serum.
Preservative≤0.1% sodium azide
CommentsAntibody specificity was examined in the rat amygdala and spinal cord. Staining is completely eliminated by pre-treatment of antibody with 10-5M rat α-CGRP and partially eliminated by preabsorption with rat β-CGRP. Preabsorption with calcitonin resulted in no loss of immunostaining. For staining paraffin sections, heat sections to 80°C in 40 mM sodium citrate buffer, pH 8.5. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Carefully reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesSudhof, T.C. 1995. Nature 375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
Application referencesParaffin Sections, Free Floating Sections, Immunofluorescence Maatkamp, A., et al. 2004. Eur. J. Neurosci. 20, 14.