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MABN396 Anti-CFDP1 Antibody, clone 15E5.1

Anti-CFDP1, clone 15E5.1, Cat. No. MABN396, is a highly specific mouse monoclonal antibody that targets CFDP1 and has been tested for use in Western Blotting.

MABN396
200 µL  
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Replacement Information
Description
Catalogue NumberMABN396
DescriptionAnti-CFDP1 Antibody, clone 15E5.1
Alternate Names
  • Bucentaur
  • CFDP1
Background InformationCraniofacial development protein 1 (UniProt: Q9UEE9; also known as Bucentaur) is encoded by the CFDP1 (also known as BCNT, CENP-29) gene (Gene ID: 10428) in human. Craniofacial development protein 1 is an evolutionarily conserved member of the Bucentaur (BCNT) family of proteins and displays ubiquitous expression. It contains a BCNT domain of about 80 amino acids in the C-terminal region. Two isoforms of craniofacial development protein 1 are reported that are generated by alternative splicing. It is reported to play a role in embryogenesis and is considered as essential for chromatin/chromosome organization and function. A unique feature of this protein is that it lacks cysteine residues and has an acidic N-terminal region and a lysine/glutamic acid/proline-rich region of 40 amino acids. It also has seven serine phosphorylation sites and Ser250 in the BCNT region is shown to be heavily phosphorylated. (Ref.: Messina, G., et al. (2015). Chromosoma 124(2):153 162; Iwashita, S et al. (2015). Biosci Rep. 35(4): e00228).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CFDP1, clone 15E5.1, Cat. No. MABN396, is a highly specific mouse monoclonal antibody that targets CFDP1 and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected CFDP1 in 10 µg of Jurkat cell lysate.
Biological Information
ImmunogenGST-taged recombinant fragment corresponding to the first 111 amino acids from the N-terminal region.
Clone15E5.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 15E5.1 detects Craniofacial development protein 1 in human. It targets an epitope within the first 111 amino acids from the N-terminal half.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CFDP1
  • BCNT
  • CENP-29
Purification MethodProtein G purified
UniProt Number
Molecular Weight~45 kDa observed; 33.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CFDP1 in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABN396 04054839337178

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