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MABT325 Anti-CEACAM5 Antibody, clone 5C8C4

Anti-CEACAM5 Antibody, clone 5C8C4 is an antibody against CEACAM5 for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-CEACAM5 Antibody, clone 5C8C4 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: CEACAM5, CEA UniProt Number: P06731
MABT325
100 µg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, FC, ICC, IH(P), IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT325
DescriptionAnti-CEACAM5 Antibody, clone 5C8C4
Alternate Names
  • CEACAM5
  • Carcinoembryonic antigen-related cell adhesion molecule 5
  • Carcinoembryonic antigen
  • CEA
  • Meconium antigen 100
  • CD66e
Background InformationThe carcinoembryonic antigen- (CEA-) related cell adhesion molecules (CEACAMs) constitute a 12-member subgroup of the CEA family of immunoglobulin-related proteins first described in 1965 (PMID 4953873). CEACAMs are reported to participate in diverse physiological processes, including cell adhesion, differentiation, proliferation, and survival, as well as in carcinogenesis and bacterial pathogenesis. CEACAM molecules from neighbouring cells can interact via their respective extracellular N-terminal IgV-like domain and mediate cell-cell adhesion through trans-oligomerization. CEACAM molecules within the same cell can also undergo transmembrane domain-mediated cis-oligomerization, an event important for sustaining downstream cellular signaling. Athough CEACAM1 is shown to utilize its cytoplasmic domain for transducing cellular signaling, not all CEACAM members are expressed with a significant cytoplasmic domain, and others (CEACAM5/6/7/8) are GPI-anchored without even a transmembrane domain. (PMID 4735478). Human CEACAM5 (also known as CD66e, CEA, Ceacam5, Meconium antigen 100; UniProt P06731) is encoded by the CEACAM5 (or CEA) gene (NCBI Gene ID 1048). It is a GPI-anchored CEACAM with the most extracellular Ig domains, 7 in all.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-CEACAM5 Antibody, clone 5C8C4 is an antibody against CEACAM5 for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CEACAM5 in human lung cancer and breast cancer tissue sections.

Immunoprecipitation Analysis: 10 µg/mL from a representative lot immunoprecipitated CEACAM5 in human granulocytes lysate (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

Western Blotting Analysis: 10 µg/mL from a representative lot detected CEACAM5 in human granulocyte lysate (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

ELISA Analysis: A representative lot detected CEACAM5 in bronchoalveolar lavage fluid and NHBE cells (Klaile, E., et al. (2013). Respir. Res. 14:85).

Flow Cytometry Analysis: A representative lot detected the exogenously expressed human CEACAM5 on the surface of transfected HeLa cells (Gutbier, B., et al. (2015). Am. J. Physiol. Lung Cell Mol. Physiol. 309(3):L250-261).

Flow Cytometry Analysis: A representative lot detected CEACAM5 in NHBE cell surfaces (Klaile, E., et al. (2013). Respir. Res. 14:85).

Immunocytochemistry Analysis: A representative lot detected CEACAM5 in non-permeabilized NHBE cells (Klaile, E., et al. (2013). Respir. Res. 14:85).

Immunohistochemistry Analysis: A representative lot detected CEACAM5 in paraffin sections of human lung tissue (Klaile, E., et al. (2013). Respir. Res. 14:85).

Western Blotting Analysis: A representative lot detected CEACAM5 in human epithelial cell (HT29, T102/3), human
endothelial cells (AS-M.5), mouse endothelial cells (bEND3), CHO and CHO-CEACAM1 cells (Muturi, H.T., et al. (2013). PLoS One. 8(9):e74654).
Biological Information
ImmunogenRecombinant human CEACAM5 extracellular domain.
Epitopeextracellular domain
Clone5C8C4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CEACAM5
  • CEA
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~180 kDa observed. Target band size appears larger than the calculated molecular weights of 71.33/76.80 kDa (mature/pro-form) due to glycosylation.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human colon cancer tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected CEACAM5 in human colon cancer tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABT325 04055977162783