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MABF444 Sigma-AldrichAnti-CD45R (B220) Antibody (human/mouse), PE-Cy5, clone RA3-6B2

This rat monoclonal antibody Anti-CD45R (B220) (human/mouse), PE-Cy5, clone RA3-6B2 validated for use in western blotting, IP, IHC, Immunofluorescence & Flow Cytometry for the detection of CD45R (

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Research Category: Inflammation & Immunology Gene Symbol: Ptprc, Ly-5 UniProt Number: P06800

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Tableau de caractéristiques principal

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	FC, IF, IHC, IP, WB	R	PE-Cy5	Monoclonal Antibody

Description
Catalogue Number	MABF444
Description	Anti-CD45R (B220) Antibody (human/mouse), PE-Cy5, clone RA3-6B2
Alternate Names	Receptor-type tyrosine-protein phosphatase C Leukocyte common antigen L-CA Lymphocyte antigen 5 Ly-5 T200 CD45
Background Information	The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.

Product Information
Format	PE-Cy5
Presentation	Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality Level	MQ100

Applications
Application	This rat monoclonal antibody Anti-CD45R (B220) (human/mouse), PE-Cy5, clone RA3-6B2 validated for use in western blotting, IP, IHC, Immunofluorescence & Flow Cytometry for the detection of CD45R (B220).
Key Applications	Flow Cytometry Immunofluorescence Immunohistochemistry Immunoprecipitation Western Blotting

Biological Information
Immunogen	Immunogen information for this antibody is proprietary information.
Clone	RA3-6B2
Concentration	0.2 mg/mL
Host	Rat
Isotype	IgG2aκ
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody
Gene Symbol	Ptprc Ly-5
Purification Method	Size Exclusion Chromatography
UniProt Number	P06800

Product Usage Statements
Quality Assurance	The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Flow Cytometry Analysis: 0.125 µg of this antibody detected CD45R (B220) in one million C57Bl/6 splenocytes.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Packaging Information
Material Size	100 µg

Global Trade Item Number
Référence	GTIN
MABF444	04053252960345

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