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MABF590 Anti-CD45.1 Antibody (mouse), APC-Cy7, clone A20

MABF590
25 µg  
Purchase on Sigma-Aldrich

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Species ReactivityKey ApplicationsHostFormatAntibody Type
MIP, IHC, FCMAPC-Cy7Monoclonal Antibody
Description
Catalogue NumberMABF590
DescriptionAnti-CD45.1 Antibody (mouse), APC-Cy7, clone A20
Alternate Names
  • Receptor-type tyrosine-protein phosphatase C
  • Leukocyte common antigen
  • L-CA
  • Lymphocyte antigen 5
  • Ly-5
  • T200
  • CD45
Background InformationThe A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.
References
Product Information
FormatAPC-Cy7
PresentationPurified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal antibody Anti-CD45.1 (mouse), APC-Cy7, clone A20 validated for use in IP, IHC & Flow Cytometry for the detection of CD45.1.
Key Applications
  • Immunoprecipitation
  • Immunohistochemistry
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneA20
Concentration0.2 mg/mL
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Ptprc
  • Ly-5
Purification MethodSize Exclusion Chromatography
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 1 μg of this antibody detected CD45.1 in one million SJL splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size25 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABF590 04053252963964