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MABF1580 Anti-CD3e (Mouse), clone 145-2C11, biotin conjugate Antibody

Anti-CD3e (Mouse), clone 145-2C11, biotin conjugate Antibody detects level of CD3e and has been published and validated for use in Flow Cytometry.

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Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: Cd3e UniProt Number: P22646
MABF1580
100 μg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFCAHmAffinity PurifiedMonoclonal Antibody
Description
Catalogue NumberMABF1580
DescriptionAnti-CD3e (Mouse), clone 145-2C11, biotin conjugate Antibody
Alternate Names
  • T-cell surface glycoprotein CD3 epsilon chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • CD3e
  • CD3e
Background InformationThe 145-2C11 antibody is specific for mouse CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The 145-2C11 antibody is a widely used phenotypic marker for mouse T cells. In addition, binding of 145-2C11 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).
References
Product Information
FormatAffinity Purified
PresentationPurified Armenian Hamster monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Quality LevelMQ100
Applications
ApplicationAnti-CD3e (Mouse), clone 145-2C11, biotin conjugate Antibody detects level of CD3e and has been published and validated for use in Flow Cytometry.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
Clone145-2C11
ConcentrationPlease refer to lot specific datasheet.
HostArmenian Hamster
IsotypeIgG
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Cd3e
Purification MethodPurified by size-exclusion chromatography
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD3e in one million C57Bl/6 splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABF1580 04055977292848