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MABF520 Anti-CD11b Antibody (human/mouse), APC, clone M1/70

This rat monoclonal antibody Anti-CD11b (human/mouse), APC & clone M1/70 validated for use in IP;IHC;Flow Cytometry;Immunofluorescence for the detection of CD11b.

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Research Category: Inflammation & Immunology Gene Symbol: ITGAM, CD11B, CR3A UniProt Number: P05555, P11215
MABF520
25 µg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MIP, IHC, FC, IFRAPCMonoclonal Antibody
Description
Catalogue NumberMABF520
DescriptionAnti-CD11b Antibody (human/mouse), APC, clone M1/70
Alternate Names
  • Integrin alpha-M
  • CD11 antigen-like family member B
  • CR-3 alpha chain
  • Cell surface glycoprotein MAC-1 subunit alpha
  • Leukocyte adhesion receptor MO1
  • Neutrophil adherence receptor
  • CD antigen CD11b
Background InformationThe M1/70 antibody reacts with human and mouse CD11b, also known as integrin alpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha M (CD11b) assembles with Integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells. The M1/70 antibody is widely used as a marker for CD11b expression on mouse macrophages, granulocytes, neutrophils, and NK cells. The antibody is also reported to be cross-reactive for Rhesus macaque CD11b.
References
Product Information
FormatAPC
PresentationPurified rat monoclonal antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rat monoclonal antibody Anti-CD11b (human/mouse), APC & clone M1/70 validated for use in IP;IHC;Flow Cytometry;Immunofluorescence for the detection of CD11b.
Key Applications
  • Immunoprecipitation
  • Immunohistochemistry
  • Flow Cytometry
  • Immunofluorescence
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneM1/70
Concentration0.2 mg/mL
HostRat
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • ITGAM
  • CD11B
  • CR3A
Purification MethodSize Exclusion Chromatography
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 µg of this antibody detected CD11b in one million C57BI/6 bone marrow cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size25 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABF520 04053252962585

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Catégories

Life Science Research > Antibodies and Assays > Primary Antibodies