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ABE1858 Anti-Bora Antibody

Anti-Bora Antibody is an antibody against Bora for use in Western Blotting, Immunoprecipitation.

Anti-Bora Antibody : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: BORA, C13orf34 UniProt Number: Q6PGQ7
ABE1858
100 µL  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPRbSerumPolyclonal Antibody
Description
Catalogue NumberABE1858
DescriptionAnti-Bora Antibody
Alternate Names
  • Protein aurora borealis
  • Bora
  • HsBora
Background InformationProtein aurora borealis (UniProt Q6PGQ7; also known as HsBora) is encoded by the BORA (also known as C13orf34) gene (Gene ID 79866) in human. Originally identified as a co-factor for Aurora A-dependent asymmetrical cell divisions, Bora is an adaptor/scaffold protein that plays an important role in mitosis by mediating Aurora A-dependent Plk1 activation, and Bora depletion abolishes both Plk1 and Cdk1 activation. Cellular Bora activity is negarively regulated by Plk1- and Cdk1-mediated phosphorylation. Cdk1 initially phosphorylates Bora on S252, which primes Bora for Plk1-catalyzed S497 and T501 phosphorylation, leading to SCFβTrCP-mediated Bora ubiquitination and subsequent proteasomal degradation. The negative feed-back regulation of Bora by the kinase it activates is important, as uncontrolled Bora overexpression perturbs Aurora A and Plk1 localization, leading to lengthened metaphase. Similar to the counter regulation between Bora and Cdk1/Plk1 in somatic cells, Bora degradation is reported to be regulated via phosphorylation by Cdk1 and Plx1 in xenopus ooctyes. Cdk1 is reported to phosphorylate Bora on Thr52, which blocks its degradation. Fertilization induced calcium surge jump-starts the oscillations of the embryonic cycles by inducing Calcineurin-catalyzed Bora pThr52 dephosphorylation, leading to Bora degradation.
References
Product Information
FormatSerum
PresentationRabbit polyclonal antibody in serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Bora Antibody is an antibody against Bora for use in Western Blotting, Immunoprecipitation.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: A representative lot detected both the endogenous Bora and a FLAG-tagged Bora exogenously expressed in HEK293T cells. Downregulation of both the endogenous and FLAG-tagged Bora was seen following replication stress induction by camptothecin (Cat. No. 208925) or UV treatment of HEK293T cells (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
Western Blotting Analysis: A representative lot detected FLAG-tagged wild-type and T501A Bora exogenously expressed in U2OS transfectants. Only the wild-type, but not the T501A mutant, was seen downregulated upon UV irradiation of the transfected U2OS cells (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
Immunoprecipitation Analysis: A representative lot immunorecipitated significantly higher amount of ubiquitinated Bora from UV-irradiated HEK293T cells pre-treated with the proteasome inhibitor MG-132 than UV-treated cells without MG-132 (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
Immunoprecipitation Analysis: A representative lot immunorecipitated Bora with enhanced Thr501 phosphorylation from UV irradiated HEK293T cells (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
Biological Information
ImmunogenGST-tagged recombinant human Bora N-terminal fragment.
EpitopeN-terminal.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antiserum reacts with unmodified as well as postranslationally modified (ubiquitination and phosphorylation) Bora (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144). Expected to react with both spliced isoforms of human Bora reported by UniProt (Q6PGQ7).
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • BORA
  • C13orf34
Purification MethodUnpurified
UniProt Number
Molecular Weight~58 kDa observed. 61.20 kDa (isoform 1) and 53.44 kDa (isoform 2) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: An 1:500 dilution of this antibody detected Bora in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
ABE1858 04055977330274