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AB2204 Sigma-AldrichAnti-BMAL1 Antibody

Anti-BMAL1 Antibody is an antibody against BMAL1 for use in WB.

Anti-BMAL1 Antibody : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

FDS
CoA
Références bibliographiques

Research Category: Neuroscience Research Sub-Category: Circadian Rhythm & Sleep Gene Symbol: BMAL1, BMAL1c, JAP3, MGC47515, MOP3, PASD3, TIC UniProt Number: O00327

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Tableau de caractéristiques principal

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB	Gp	Serum	Polyclonal Antibody

Description
Catalogue Number	AB2204
Description	Anti-BMAL1 Antibody
Alternate Names	ARNT-like protein 1, brain and muscle Basic-helix-loop-helix-PAS protein MOP3 Brain and muscle ARNT-like 1, member of PAS protein 3 aryl hydrocarbon receptor nuclear translocator-like bHLH-PAS protein JAP3 basic-helix-loop-helix-PAS orphan MOP3 2, member of PAS superfamily 3
Background Information	Brain and muscle Arnt-like protein-1 (BMAL1; also known as MOP3 or Arnt3) is a transcription factor known to regulate circadian rhythm. BMAL1 is the only component of the mammalian circadian clock whose sole deletion in a mouse model generates arrhythmicity. In addition to defects in the clock, these BMAL1 null-mice also have reproductive problems are small in stature, age quickly and have progressive arthropathy that results in having less overall locomotor activity than wild type mice. Recent phenotyping data suggests that BMAL1 and its partner Clock also play a role in regulation of glucose homeostasis and metabolism. Finally, BMAL1, NPAS2, and PER2 have been associated with seasonal affective disorder in humans. BMAL1 transcription is circadian and reciprocally regulated by NR1D1 (Rev-erb-alpha) and RORA, which establishes a second interlocking loop in the mammalian circadian clock.

References

Product Information
Format	Serum
Control	C2C12 Tissue lysate
Presentation	Serum with 0.05% NaN3
Quality Level	MQ100

Applications
Application	Anti-BMAL1 Antibody is an antibody against BMAL1 for use in WB.
Key Applications	Western Blotting

Biological Information
Immunogen	Recombinant Protein
Epitope	C-Terminus
Host	Guinea Pig
Specificity	Cat. # AB2204 will recognize the C-terminus of BMAL1.
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Mouse, Human and Rat. Reactivity with other species has not been tested.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_031515
Gene Symbol	BMAL1 BMAL1c JAP3 MGC47515 MOP3 PASD3 TIC
UniProt Number	O00327
UniProt Summary	FUNCTION: ARNTL-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins (By similarity). SUBUNIT: Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with CLOCK is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Interacts with HSP90; with AHR in vitro, but not in vivo. SUBCELLULAR LOCATION: Nucleus (By similarity). ALTERNATIVE PRODUCTS: 8 named isoforms [FASTA] produced by alternative splicing. Additional isoforms seem to exist.
Molecular Weight	~69 kDa.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely tested on C2C12 tissue lysate Lot Specific Tested Application 1: Western Blot
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt.

Packaging Information
Material Size	50 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Référence	GTIN
AB2204	04053252270703

Documentation

Anti-BMAL1 Antibody FDS

Titre
Fiche de données de sécurité des matériaux (FDS)

Anti-BMAL1 Antibody Certificats d'analyse

Titre	Numéro de lot
Anti-BMAL1 - 2455610	2455610
Anti-BMAL1 - 2060991	2060991
Anti-BMAL1 - 2246845	2246845
Anti-BMAL1 - 2298435	2298435
Anti-BMAL1 - 3290800	3290800
Anti-BMAL1 - 3437954	3437954
Anti-BMAL1 - 3448839	3448839
Anti-BMAL1 - 3722056	3722056
Anti-BMAL1 - 3805294	3805294
Anti-BMAL1 - 3958305	3958305

Références bibliographiques

Aperçu de la référence bibliographique	Nº PubMed
Antibodies for assessing circadian clock proteins in the rodent suprachiasmatic nucleus. LeSauter, J; Lambert, CM; Robotham, MR; Model, Z; Silver, R; Weaver, DR PloS one 7 e35938 2011 Afficher le résumé Research on the mechanisms underlying circadian rhythmicity and the response of brain and body clocks to environmental and physiological challenges requires assessing levels of circadian clock proteins. Too often, however, it is difficult to acquire antibodies that specifically and reliably label these proteins. Many of these antibodies also lack appropriate validation. The goal of this project was to generate and characterize antibodies against several circadian clock proteins. We examined mice and hamsters at peak and trough times of clock protein expression in the suprachiasmatic nucleus (SCN). In addition, we confirmed specificity by testing the antibodies on mice with targeted disruption of the relevant genes. Our results identify antibodies against PER1, PER2, BMAL1 and CLOCK that are useful for assessing circadian clock proteins in the SCN by immunocytochemistry.	22558277
Mop3 is an essential component of the master circadian pacemaker in mammals. Bunger, M K, et al. Cell, 103: 1009-17 (2000) 1999 Afficher le résumé Circadian oscillations in mammalian physiology and behavior are regulated by an endogenous biological clock. Here we show that loss of the PAS protein MOP3 (also known as BMAL1) in mice results in immediate and complete loss of circadian rhythmicity in constant darkness. Additionally, locomotor activity in light-dark (LD) cycles is impaired and activity levels are reduced in Mop3-/- mice. Analysis of Period gene expression in the suprachiasmatic nucleus (SCN) indicates that these behavioral phenotypes arise from loss of circadian function at the molecular level. These results provide genetic evidence that MOP3 is the bona fide heterodimeric partner of mCLOCK. Furthermore, these data demonstrate that MOP3 is a nonredundant and essential component of the circadian pacemaker in mammals.	11163178
Rapid resetting of the mammalian circadian clock. Best, J D, et al. J. Neurosci., 19: 828-35 (1999) 1998	9880602

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Catégories

Life Science Research > Antibodies and Assays > Primary Antibodies

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