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GF35L Anti-BDNF Mouse mAb (35928.11)

GF35L
Purchase on Sigma-Aldrich

Aperçu

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

RéférenceConditionnement Qté
GF35L-100UG Ampoule plast. 100 μg
Description
OverviewRecognizes the ~20 kDa BDNF protein in brain extracts. Clone 35928.11 was selected based on its ability to neutralize the biological activity of human recombinant BDNF.
Catalogue NumberGF35L
Brand Family Calbiochem®
SynonymsAnti-Brain Derived Neurotrophic Factor
References
ReferencesRiccio, A., et al. 1997. Science 277 1097.
Sendtner, M., et al. 1996. Neurochem. Res. 21, 831.
Barbacid, M. 1995. Ann. N.Y. Acad. Sci. 766, 442.
Gotz, R., et al. 1994. Nature 372, 266.
Snider, W.D. 1994. Cell 77, 627.
Snider, W.D., et al. 1992. J. Neurobiol. 23, 1231.
Product Information
FormLyophilized
FormulationLyophilized from PBS, 5% trehalose.
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunohistochemistry
Neutralization Studies
Application NotesELISA (0.5-1 µg/ml)
Immunoblotting (1-2 µg/ml)
Immunohistochemistry (5-15 µg/ml)
Neutralization Studies (see comments)
Application CommentsThe full-length sequence for BDNF can be found under accession number NP_001700. This antibody has been selected for its ability to neutralize the biological activity of human recombinant BDNF. The exact concentration of antibody required to neutralize human recombinant BDNF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of BDNF activity is approximately 5-15 µg/ml in the presence of 2.5 ng/ml of human recombinant BDNF, in a neuron survival assay using embryonic chick dorsal root ganglia neurons. The detection limit for human recombinant BDNF is ~300 ng/lane under non-reducing and reducing conditions. The detection for human recombinant BDNF is ~12.5 ng/ml. Based on immunoblotting and ELISA, this antibody exhibits less than 2% cross-reactivity with human recombinant β-NGF, human recombinant NT-3, and human recombinant NT-4. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant protein consisting of amino acids 129-247 of human BDNF
ImmunogenHuman
Clone35928.11
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, aliquot and freeze (-20°C).
Global Trade Item Number
Référence GTIN
GF35L-100UG 04055977220124