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ABT251 Anti-Arpin Antibody

This Anti-Arpin Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of Arpin.

Anti-Arpin Antibody : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: ARPIN, C15orf38 UniProt Number: Q7Z6K5
ABT251
100 µL  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT251
DescriptionAnti-Arpin Antibody
Alternate Names
  • Arp2/3 inhibition protein
  • Arpin
Background InformationArpin (UniProt Q7Z6K5; also known as Arp2/3 inhibition protein, UPF0552 protein C15orf38) is encoded by the ARPIN (also known as C15orf38) gene (Gene ID 348110) in human. Cell migration requires the generation of branched actin networks that power the protrusion of the plasma membrane in lamellipodia. The actin-related proteins 2 and 3 (Arp2/3) complex nucleates actin filaments by binding to the side of an existing filament and initiating branch formation. Arpin functions as a negative regulator of Arp2/3 activity, allowing cells to control directional persistence of migration by fine-tuning actin nucleation activity at the leading edge of the lamellipodium. Arpin depletion in both mammalian cells and Dictyostelium discoideum amoeba is reported to result in straighter trajectories. Arpin microinjection is demonstrated to induce fish keratocytes to turn.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Arpin Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of Arpin.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected detected Arpin and C15orf38-AP3S2 in MEF-1 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Arpin near the C-terminus.
Epitopenear Arpin C-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityReacts with both Arpin and the C15orf38-AP3S2 fusion (isoform 2) produced from a naturally occurring readthrough transcript.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Chimpanzee, Canine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ARPIN
  • C15orf38
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~28/42 kDa observed (24.9 kDa/Arpin and 43.9 kDa/C15orf38-AP3S2 calculated)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MEF-1 murine embryonic fibroblasts cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected detected Arpin and C15orf38-AP3S2 in 10 µg of human U2OS and murine MEF-1 embryonic fibroblasts cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
ABT251 04055977274912