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MABS1310-100UL Anti-Apolipoprotein A-I Antibody, clone Wt20-7

Anti-Apolipoprotein A-I, clone Wt20-7, Cat. No. MABS1310, is a mouse monoclonal antibody that detects human Apolipoprotein A-I and has been tested for use in Dot Blot, ELISA, Immunocytochemistry, and Western Blotting.

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Research Category: Signaling Gene Symbol: APOA1 UniProt Number: P02647
MABS1310-100UL
100 µL  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HDB, ELISA, ICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1310-100UL
DescriptionAnti-Apolipoprotein A-I Antibody, clone Wt20-7
Alternate Names
  • Apo-AI
  • ApoA-I
  • Apolipoprotein A1
Background InformationApolipoprotein A-I (UniProt: P02647; also known as Apo-AI, ApoA-I, Apolipoprotein A1) is encoded by the APOA1 gene (Gene ID: 335) in human. Apo A-1 is a major protein of plasma high density lipoprotein (HDL) and is also found in chylomicrons. It plays a major role in transporting cholesterol from peripheral tissues to the liver. It is synthesized mainly in the liver with a signal peptide (aa 1-18), which is proteolytically cleaved to generate the mature protein. Apo A-1 also serves as a co-factor for lecithin cholesterolacyltransferase (LCAT) that is involved in the formation of cholesteryl esters. Elevated levels of oxidized Apo A-1 (on Methionine 110 and 136) have been linked to increased risk for coronary artery disease. Mutations in APOA1 gene have also been linked to HDL deficiencies that lead to premature coronary artery disease and a reduction in cellular cholesterol efflux. Mutations in APOA1 gene have also shown to result in amyloidosis with extensive visceral amyloid deposit and in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as familial amyloid polyneuropathy type III.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Apolipoprotein A-I, clone Wt20-7, Cat. No. MABS1310, is a mouse monoclonal antibody that detects human Apolipoprotein A-I and has been tested for use in Dot Blot, ELISA, Immunocytochemistry, and Western Blotting.
Key Applications
  • Dot Blot
  • ELISA
  • Immunocytochemistry
  • Western Blotting
Application NotesELISA Analysis: A representative lot detected Apolipoprotein A-I in ELISA applications (Kimura, H., et. al. (2017). Sci Rep. 7(1):2988).

Western Blotting Analysis: A representative lot detected Apolipoprotein A-I in Western Blotting applications (Kuwabara, K., et. al. (2015). J Biol Chem. 290(40):24210-21).

Immunocytochemistry Analysis: A representative lot detected Apolipoprotein A-I in Immunocytochemistry applications (Kameyama, H., et. al. (2016). Sci Rep. 6:30391; Kuwabara, K., et. al. (2015). J Biol Chem. 290(40):24210-21).

Dot Blot Analysis: A representative lot detected Apolipoprotein A-I in Dot Blot applications (Kimura, H., et. al. (2017). Sci Rep. 7(1):2988; Kameyama, H., et. al. (2016). Sci Rep. 6:30391; Kuwabara, K., et. al. (2015). J Biol Chem. 290(40):24210-21).
Biological Information
ImmunogenGST-tagged full-length human recombinant Apolipoprotein A-I
CloneWt20-7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone Wt20-7 specifically detects human Apolipoprotein A-I.
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • APOA1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~28 kDa observed; 30.78 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human fetal liver tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Apolipoprotein A-I in human fetal liver tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABS1310-100UL 04054839519376