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ABT260 Anti-Amelogenin, X isoform/AMELX Antibody

ABT260
100 µL  
Purchase on Sigma-Aldrich

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Aperçu

Replacement Information

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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, PorcineWB, IHCRbSerumPolyclonal Antibody
Description
Catalogue NumberABT260
DescriptionAnti-Amelogenin, X isoform/AMELX Antibody
Alternate Names
  • Amelogenin, X isoform
  • Leucine-rich amelogenin peptide
  • LRAP
Background InformationAmelogenin, X isoform/AMELX, also known as Leucine-rich Amelogenin peptide, or LRAP, and encoded by the gene AMELX/AMG/AMGX, is an important protein in the biomineralization of teeth. AMELX appears to regulate the formation of crystallites during the secretory stage of tooth enamel development. AMELX is a secreted extracellular matrix protein that has many isoforms. Interestingly mutations in the AMELX protein are associated with changes in enamel microstructure that are linked with severe dental disease, and the various isoforms of AMELX protein all aid in increasing enamel thickness of the developing tooth.
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Amelogenin, X isoform/AMELX Antibody is an antibody against Amelogenin for use in western blotting & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: A 1:1,000 dilution of this antibody detected Amelogenin, X isoform/AMELX using recombinant mouse Amelogenin179.

Western Blotting Analysis: A representative lot from an independent laboratory detected Amelogenin, X isoform/AMELX in DESC spheres and cervical loop lysates (Chang, J. Y., et al. (2013). J Cell Biol. 288(40):28952-289561.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Amelogenin, X isoform/AMELX in DESC spheres and cervical loops (Chang, J. Y., et al. (2013). J Cell Biol. 288(40):28952-289561.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Amelogenin, X isoform/AMELX in normal ameloblasts in the wild type and the pseudostratified layer and amorphous matrix of the null mice (Rios, H., et al. (2005). Mol Cell. Biol. 25(24):11131-11144.).
Biological Information
ImmunogenRecombinant protein corresponding to mouse Amelogenin, X isoform/AMELX.
HostRabbit
Species Reactivity
  • Mouse
  • Porcine
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Amelx
  • Amel
Purification MethodUnpurified
UniProt Number
Molecular Weight~23 kDa observed. At least 3 isoforms may be observed between 19-23 kDa in pig enamel acid extracts (Chang, J. Y., et al. (2013). J Cell Biol. 288(40):28952-289561.). Uncharacterized band(s) may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in pig enamel acid extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Amelogenin, X isoform/AMELX in pig enamel acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
ABT260 04053252976872

Documentation

Anti-Amelogenin, X isoform/AMELX Antibody FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-Amelogenin, X isoform/AMELX Antibody Certificats d'analyse

TitreNuméro de lot
Anti-Amelogenin, X isoform/AMELX - Q2430179 Q2430179
Anti-Amelogenin, X isoform/AMELX - 3267284 3267284
Anti-Amelogenin, X isoform/AMELX - 3860854 3860854
Anti-Amelogenin, X isoform/AMELX - 4037080 4037080
Anti-Amelogenin, X isoform/AMELX - 4068637 4068637
Anti-Amelogenin, X isoform/AMELX -2885161 2885161
Anti-Amelogenin, X isoform/AMELX Polyclonal Antibody 2945577

Références bibliographiques

Aperçu de la référence bibliographiqueNº PubMed
Fibroblast growth factor signaling is essential for self-renewal of dental epithelial stem cells.
Chang, Julia Yu Fong, et al.
J. Biol. Chem., 288: 28952-61 (2013)  2013

Afficher le résumé
23979135 23979135
periostin null mice exhibit dwarfism, incisor enamel defects, and an early-onset periodontal disease-like phenotype.
Rios, Hector, et al.
Mol. Cell. Biol., 25: 11131-44 (2005)  2004

Afficher le résumé
16314533 16314533