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MABF31 Anti-Alpha Defensin-5 (HD5) Antibody, clone 8C8

Anti-Alpha Defensin-5 (HD5) Antibody, clone 8C8 detects level of Alpha Defensin-5 (HD5) & has been published & validated for use in IH & WB.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: DEF5, DEFA5, HD-5 UniProt Number: Q01523
MABF31
100 µg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIHC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF31
DescriptionAnti-Alpha Defensin-5 (HD5) Antibody, clone 8C8
Alternate Names
  • defensin, alpha 5, Paneth cell-specific
  • defensin, alpha 5, preproprotein
  • defensin-5
  • defensin 5
  • Defensin, alpha 5
Background InformationHuman alpha defensin-5 (HD5) is one of six peptides belonging to the alpha defensin family. Defensins are small peptides involved in immunological defense against viral and microbial infections through either direct interaction with pathogens or in combination with other immune system factors. There are three subfamilies of defensins (α, β, and θ). α defensins are only observed in mammals and are mainly β-sheet structures. Alpha defensin-5 (HD5) is primarily expressed in the Paneth cells found within the small intestine, and has been shown to possess activity against both Gram-positive and Gram-negative microbes. Studies have shown alpha defensin-5 (HD5) to be a strong antagonist towards human Papillomavirus infection. It may also play a role Crohn’s disease.
References
Product Information
FormatPurified
Control
  • Normal human tranverse colon tissue
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Alpha Defensin-5 (HD5) Antibody, clone 8C8 detects level of Alpha Defensin-5 (HD5) & has been published & validated for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern Blot Analysis: 0.5 ug/mL from a previous lot detected alpha defensin-5 (HD5) in human small intestine tissue lysate. The urea-treated method is recommended instead of SDS page (Shen, B. et al., 2005).
Biological Information
ImmunogenRecombinant protein corresponding to human alpha defensin-5 (HD5).
Clone8C8
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryDefensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq].
Gene Symbol
  • DEF5
  • DEFA5
  • HD-5
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.

SUBUNIT STRUCTURE: Homodimer.

SUBCELLULAR LOCATION: Secreted.

TISSUE SPECIFICTY: Paneth cells of the small intestine.

SEQUENCE SIMILARITIES: Belongs to the alpha-defensin family.
Molecular Weight10 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in normal human tranverse colon tissue.

Immunohistochemistry Analysis: A 1:200 dilution of this antibody detected alpha defensin-5 (HD5) in normal human tranverse colon tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MABF31 04053252680854

Documentation

Anti-Alpha Defensin-5 (HD5) Antibody, clone 8C8 FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-Alpha Defensin-5 (HD5) Antibody, clone 8C8 Certificats d'analyse

TitreNuméro de lot
Anti-Alpha Defensin-5 (HD5), -2821652 2821652
Anti-Alpha Defensin-5 (HD5), clone 8C8 - 2194100 2194100
Anti-Alpha Defensin-5 (HD5), clone 8C8 - 2197236 2197236
Anti-Alpha Defensin-5 (HD5), clone 8C8 - 2199662 2199662
Anti-Alpha Defensin-5 (HD5), clone 8C8 - 3597395 3597395
Anti-Alpha Defensin-5 (HD5), clone 8C8 - 3942184 3942184
Anti-Alpha Defensin-5 (HD5), clone 8C8 - 4198596 4198596
Anti-Alpha Defensin-5 (HD5), clone 8C8 - NRG1784845 NRG1784845
Anti-Alpha Defensin-5 (HD5), clone 8C8 Monoclonal Antibody 2941519