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ABC137 Anti-ATPIF1 Antibody

This Anti-ATPIF1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of ATPIF1.

Anti-ATPIF1 Antibody : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: ATPIF1, ATPI UniProt Number: Q9UII2
ABC137
100 µg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC137
DescriptionAnti-ATPIF1 Antibody
Alternate Names
  • ATPase inhibitor, mitochondrial
  • Inhibitor of F(1)F(o)-ATPase
  • IF(1)
  • IF1
  • ATPIF1
Background InformationATPIF1 is an interesting protein that acts on ATP synthase and prevents it from catabolizing ATP when the mitochondrial membrane potential falls below a threshold, which ATP synthase starts to do by hydrolyzing ATP to pump protons out of the mitochondrial matrix to restore ion balance. ATPIF1 also indirectly regulates heme synthesis by modulating the mitochondrial pH and redox potential. ATPIF1 is expressed in the mitochondria and its active is determined by conformation. Homodimers are active, homotetramers are inactive and their formation is governed by pH. ATPIF1, because of its effects on ATP and heme, is important in cellular development, heme biosynthesis, energy metabolism and angiogenesis and erythrocytes differentiation.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-ATPIF1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of ATPIF1.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 2.0 µg/mL from a representative lot detected ATPIF1 in 10 µg of HEK293 cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected ATPIF1 in HeLa, A431, and NIH/3T3 cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the N-terminal inhibitory region & Antiparallel alpha-helical coiled eegion of human ATPIF1.
EpitopeN-terminal inhibitory region & Antiparallel alpha-helical coiled region
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ATPIF1
  • ATPI
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~12 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in T47D cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected ATPIF1 in 10 µg of T47D cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
ABC137 04055977073171