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ABE2614 Anti-ATF5

Anti-ATF5, Cat. No. ABE2614, is a highly specific rabbit polyclonal antibody that targets Cyclic AMP-dependent transcription factor ATF-5 and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Anti-ATF5 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Gene Symbol: ATFS, ATFX UniProt Number: Q9Y2D1
ABE2614
100 µL  
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Replacement Information

Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
Mk, R, HIF, ICC, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABE2614
DescriptionAnti-ATF5
Alternate Names
  • Cyclic AMP-dependent transcription factor ATF-5
  • cAMP-dependent transcription factor ATF-5
  • Activating transcription factor 5
  • Transcription factor ATFx
Background InformationCyclic AMP-dependent transcription factor ATF-5 (UniProt: also known as cAMP-dependent transcription factor ATF-5, Activating transcription factor 5, Transcription factor ATFx) is encoded by the ATF5 (also known as ATFX) gene (Gene ID: 22809) in human. ATF5 belongs to the ATF/CREB family of transcription factors that is widely expressed. It has been implicated in multiple biological processes, such as regulation of cell proliferation and differentiation, cell stress response and survival. ATF5 can either stimulate or repress gene transcription through binding of different DNA regulatory elements such as CRE (cAMP response element), ARE (ATF5 regulatory element), and AARE (amino acid response element). Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. ATF5 is an essential structural protein that is required for the interaction between the mother centriole and the pericentriolar material (PCM). ATF5 depletion is reported to block PCM accumulation at the centrosome and cause fragmentation of centrioles, which contributes to the formation of multi-polar mitotic spindles and genomic instability. ATF5 homeostasis is essential for the function of the centrosome and for the global microtubule organization.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-ATF5, Cat. No. ABE2614, is a highly specific rabbit polyclonal antibody that targets Cyclic AMP-dependent transcription factor ATF-5 and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected ATF5 in 10 µg of C6 cell lysate.

Western Blotting Analysis: A representative lot detected ATF5 in Western Blot applications. (Madarampalli, B., et. al. (2015). Cell. 162(3):580-92).

Immunofluorescence Analysis: A representative lot detected ATF5 in Immunofluorescence applications. (Madarampalli, B., et. al. (2015). Cell. 162(3):580-92).

Western Blotting Analysis: A representative lot detected ATF5 in transfected Hela Cells (Courtesy of Dr. David X. Liu from Washington State University).

Immunofluorescence Analysis: A representative lot detected ATF5 in Cos-7, Hela, and C6 cells (Courtesy of Dr. David X. Liu from Washington State University).
Biological Information
ImmunogenA linear peptide corresponding to 18 amino acids from the N-terminal half of human Cyclic AMP-dependent transcription factor ATF-5.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody targets a sequence in the N-terminal region and detects ATF5 by immunocytochemstry.
Species Reactivity
  • Monkey
  • Rat
  • Human
Species Reactivity NoteHuman, Monkey, Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ATFS
  • ATFX
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~32 kDa observed; 30.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in COS-7 cells.

Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected ATF5 in COS-7 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
ABE2614 04054839156458