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MAB348-AF647 Anti-APP A4, a.a. 66-81 of APP {NT} Antibody, clone 22C11, Alexa Fluor® 647 Conjugate

Anti-APP A4, a.a. 66-81 of APP {NT} Antibody, clone 22C11, Alexa Fluor® 647 Conjugate is an antibody against APP A4 for use in Immunocytochemistry.

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Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: APP, A4, AD1 UniProt Number: P05067
MAB348-AF647
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Species ReactivityKey ApplicationsHostFormatAntibody Type
F, Ca, H, M, R, Porcine, MkICCMAlexaFluor®647Monoclonal Antibody
Description
Catalogue NumberMAB348-AF647
DescriptionAnti-APP A4, a.a. 66-81 of APP {NT} Antibody, clone 22C11, Alexa Fluor® 647 Conjugate
Alternate Names
  • Amyloid beta A4 protein
  • ABPP
  • Alzheimer disease amyloid protein
  • Amyloid precursor protein
  • APP
  • APPI
  • Cerebral vascular amyloid peptide
  • CVAP
  • PN-II
  • PreA4
  • Protease nexin-II
  • PN-II
  • N-APP
  • Soluble APP-alpha
  • S-APP-alpha
  • Soluble APP-beta
  • S-APP-beta
  • C99
  • Beta-amyloid protein 42
  • Beta-APP42
  • Beta-amyloid protein 40
  • Beta-APP40
  • C83
  • P3(42)
  • P3(40)
  • C80
  • Gamma-secretase C-terminal fragment 59
  • Amyloid intracellular domain 59
  • AICD-59
  • AID(59)
  • Gamma-CTF(59)
  • Gamma-secretase C-terminal fragment 57
  • Amyloid intracellular domain 57
  • AICD-57
  • AID(57)
  • Gamma-CTF(57)
  • Gamma-secretase C-terminal fragment 50
  • Amyloid intracellular domain 50
  • AICD-50
  • AID(50)
  • Gamma-CTF(50)
  • C31
Background InformationAmyloid beta A4 protein (UniProt P05067; also known as ABPP, Alzheimer disease amyloid protein, Amyloid precursor protein, APP, APPI, Cerebral vascular amyloid peptide, CVAP, PN-II, PreA4, Protease nexin-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID 351) in human. Amyloid precursor protein (APP) is initially produced with a signal peptide sequence (a.a. 1-17), the removal of which yields the mature protein with a large extracellular portion (a.a. 18-699), followed by a transmembrane segment (a.a. 700-723) and a cytoplasmic (a.a. 724-770) tail. APP can be further processed by the α-, β-, and γ-secretases in two alternative processing pathways. In the non-amyloidogenic pathway, APP is first cleaved by the plasma membrane-localized α-secretase to generate an N-terminal extracellular sAPPα fragment (a.a. 18-687) and a membrane-bound C-terminal fragment C83 (CTFα), which can be further cleaved by γ-secretase to produce a non-toxic small peptide p3 and a cytoplasmic APP intracellular domain (AICD). In the amyloidogenic pathway, APP undergoes β-cleavage in BACE-1 (β-site APP-cleaving enzyme)-enriched endosomes to generate an N-terminal extracellular sAPPβ fragment (a.a. 18-671) and a membrane-bound C-terminal fragment C99 (CTFβ). Subsequent cleavage of C99 by γ-secretase releases the amyloid β peptide (Aβ) and AICD. Aβ accumulation in the cortical and hippocampal regions of the brain is a major pathological feature of Alzheimer's disease (AD).
References
Product Information
FormatAlexaFluor®647
PresentationPurified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-APP A4, a.a. 66-81 of APP {NT} Antibody, clone 22C11, Alexa Fluor® 647 Conjugate is an antibody against APP A4 for use in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesThe unconjugated antibody (Cat. No. MAB348) is shown to be suitable also for immunohistochemistry (for both frozen and paraffin-embedded sections), immunofluorescence, and Western blotting applications.
Biological Information
ImmunogenPurified recombinant Alzheimer precursor A4 (pre-A4695) fusion protein.
EpitopeAmino acids 66-81 of APP.
Clone22C11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 22C11 reacts with pre-A4. The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich, C., et al. (1993). J. Biol. Chem. 268(35):26571-26577). Clone 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann, J., et al. (2000). Eur. J. Cell. Biol.79(12):905-914.). The antibody is known to cross react with APLP2 (Slunt, H.H., et al. (1994). J. Biol. Chem. 269(4):2637-2644).
IsotypeIgG1
Species Reactivity
  • Fish
  • Canine
  • Human
  • Mouse
  • Rat
  • Porcine
  • Monkey
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • APP
  • A4
  • AD1
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~110/120/130 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in SH-SY5Y cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected APP A4 in SH-SY5Y cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MAB348-AF647 04055977311983

Documentation

Anti-APP A4, a.a. 66-81 of APP {NT} Antibody, clone 22C11, Alexa Fluor® 647 Conjugate FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-APP A4, a.a. 66-81 of APP {NT} Antibody, clone 22C11, Alexa Fluor® 647 Conjugate Certificats d'analyse

TitreNuméro de lot
Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 647 Conjugate - 3036826 3036826
Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 647 Conjugate - 3160113 3160113
Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 647 Conjugate - 3202182 3202182
Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 647 Conjugate - 3422831 3422831
Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 647 Conjugate - 3592154 3592154
Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 647 Conjugate - 4023280 4023280
Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 647 Conjugate - 4079017 4079017
Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 647 Conjugate - 4149897 4149897
Anti-APP A4, a.a. 66-81 of APP {NT}, clone 22C11, Alexa Fluor® 647 Conjugate -Q2637234 Q2637234

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Titre
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