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NE1037 Anti-APOC1 Mouse mAb (2E2-1A3)

NE1037
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

RéférenceConditionnement Qté
NE1037-100UG 100 μg
Description
OverviewRecognizes the ~10 kDa APOC1 protein in human liver tissue.
Catalogue NumberNE1037
Brand Family Calbiochem®
SynonymsAnti-Apolipoprotein
Application Data
Detection of human APOC1 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-APOC1 Mouse mAb (2E2-1A3) (Cat. No. NE1037) (1-5 µg/ml). Detection: chemiluminescence.
References
ReferencesAndersson, B., et al. 2009. Proteome Sci. 7, 40.
Meunier, J.C., et al. 2008. J. Virol.<,i> 82 9647.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHuman liver tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 1-84 of human APOC1, expressed as a GST fusion protein
Clone2E2-1A3
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
NE1037-100UG 04055977209938

Documentation

Anti-APOC1 Mouse mAb (2E2-1A3) FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Références bibliographiques

Aperçu de la référence bibliographique
Andersson, B., et al. 2009. Proteome Sci. 7, 40.
Meunier, J.C., et al. 2008. J. Virol.<,i> 82 9647.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-November-2010 RFH
SynonymsAnti-Apolipoprotein
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human APOC1 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-APOC1 Mouse mAb (2E2-1A3) (Cat. No. NE1037) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~10 kDa APOC1 protein.
BackgroundAPOC1 is a member of the apolipoprotein C1 family. This protein is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. This gene is mapped to chromosome 19, where it resides within an apolipoprotein gene cluster. Alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 1-84 of human APOC1, expressed as a GST fusion protein
Clone2E2-1A3
IsotypeIgG₁
Specieshuman
Positive controlHuman liver tissue
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesAndersson, B., et al. 2009. Proteome Sci. 7, 40.
Meunier, J.C., et al. 2008. J. Virol.<,i> 82 9647.