Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MAB3786 Anti-APC Antibody, CT, clone C-APC 28.9

Anti-APC Antibody, C-terminus, clone C-APC 28.9 is a Mouse Monoclonal Antibody for detection of APC also known as Adenomatous Polyposis Coli Protein & has been validated in IF, IP, WB & IHC.

Anti-APC Antibody, CT, clone C-APC 28.9 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: APC, DP2.5, FAP, GS, DP3, DP2, FPC UniProt Number: P25054
MAB3786
100 µg  
Purchase on Sigma-Aldrich

Offres spéciales

Aperçu

Replacement Information

Offres spéciales

100% Performance Guaranteed

Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MIF, IP, WB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3786
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-APC Antibody, CT, clone C-APC 28.9
Alternate Names
  • Adenomatous Polyposis Coli Protein
Background InformationThe adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. C-APC 28.8 can be used for APC expression and detection of APC mutations.
References
Product Information
FormatPurified
Control
  • POSITIVE CONTROL: colon cell line HCT116
PresentationPurified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-APC Antibody, C-terminus, clone C-APC 28.9 is a Mouse Monoclonal Antibody for detection of APC also known as Adenomatous Polyposis Coli Protein & has been validated in IF, IP, WB & IHC.
Key Applications
  • Immunofluorescence
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern blot

Immunohistochemistry (Frozen sections)

Immunofluorescence

Immunoprecipitation

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenHuman APC C-terminal fusion with MBP (maltose binding protein).
EpitopeC-terminus
CloneC-APC 28.9
HostMouse
SpecificityRecognizes human APC, molecular weight is approximately 310kDa.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
Gene Symbol
  • APC
  • DP2.5
  • FAP
  • GS
  • DP3
  • DP2
  • FPC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P25054 # Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. APC activity is correlated with its phosphorylation state.
SIZE: 2843 amino acids; 311646 Da
SUBUNIT: Forms homooligomers. Interacts with DIAPH1 and DIAPH2 (By similarity). Interacts with PDZ domains of DLG1 and DLG3. Associates with catenins. Binds axin. Interacts with the N- terminus of ARHGEF4, and the C-terminus of MAPRE1, MAPRE2 and MAPRE3. Found in a complex consisting of ARHGEF4, APC and CTNNB1.
TISSUE SPECIFICITY: Expressed in a variety of tissues.
PTM: Phosphorylated by GSK3B.
DISEASE: SwissProt: P25054 # Defects in APC are a cause of familial adenomatous polyposis (FAP) [MIM:175100]; which includes also Gardner syndrome (GS). FAP and GS contribute to tumor development in patients with uninherited forms of colorectal cancer. FAP is characterized by adenomatous polyps of the colon and rectum, but also of upper gastrointestinal tract (ampullary, duodenal and gastric adenomas). This is a viciously premalignant disease with one or more polyps progressing through dysplasia to malignancy in untreated gene carriers with a median age at diagnosis of 40 years. & APC mutations have led to some interesting observations. (1) the great majority of the mutations found to date would result in truncation of the APC product. (2) almost all the mutations have occurred within the first half of the coding sequence, and somatic mutations in colorectal tumors are further clustered in a particular region, called MCR (mutation cluster region). (3) most identified point mutations in the APC gene are transitions from cytosine to other nucleotides. (4) the location of germline mutations tends to correlate with the number of colorectal polyps in FAP patients. Inactivation of both alleles of the APC gene seems to be required as an early event to develop most adenomas and carcinomas in the colon and rectum as well as some of those in the stomach. & Defects in APC are a cause of hereditary desmoid disease (HDD) [MIM:135290]; also called familial infiltrative fibromatosis (FIF). It is an autosomal dominant trait with 100% penetrance and possible variable expression among affected relatives. HDD patients show multifocal fibromatosis of the paraspinal muscles, breast, occiput, arms, lower ribs, abdominal wall, and mesentery. Desmoid tumors appears also as a complication of familial adenomatous polyposis. & Defects in APC are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Although the majority of medulloblastomas occur sporadically, some manifest within familial cancer syndromes such as Turcot syndrome and basal cell nevus syndrome (Gorlin syndrome). & Defects in APC are a cause of Turcot syndrome [MIM:276300]. Turcot syndrome is an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots.
SIMILARITY: SwissProt: P25054 ## Contains 7 ARM repeats.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
MAB3786 04053252723834

Documentation

Anti-APC Antibody, CT, clone C-APC 28.9 FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Anti-APC Antibody, CT, clone C-APC 28.9 Certificats d'analyse

TitreNuméro de lot
Anti-APC CT, clone C-APC 28.9 MONOCLONAL ANTIBODY Q2922309
MOUSE ANTI-HUMAN APC (C-TERMINAL) MONOCLONAL ANTIBODY - 2391077 2391077
MOUSE ANTI-HUMAN APC (C-TERMINAL) - 2554100 2554100
MOUSE ANTI-HUMAN APC (C-TERMINAL) - 3284325 3284325
MOUSE ANTI-HUMAN APC (C-TERMINAL) - 3318921 3318921
MOUSE ANTI-HUMAN APC (C-TERMINAL) - 3384539 3384539
MOUSE ANTI-HUMAN APC (C-TERMINAL) - 4189665 4189665
MOUSE ANTI-HUMAN APC (C-TERMINAL) -2688643 2688643
MOUSE ANTI-HUMAN APC (C-TERMINAL) -2739323 2739323
MOUSE ANTI-HUMAN APC (C-TERMINAL) -2789778 2789778
Show more

Références bibliographiques

Aperçu de la référence bibliographiqueApplicationEspèceNº PubMed
17β-estradiol protects human eyelid-derived adipose stem cells against cytotoxicity and increases transplanted cell survival in spinal cord injury.
Zhou, J; Lu, P; Ren, H; Zheng, Z; Ji, J; Liu, H; Jiang, F; Ling, S; Heng, BC; Hu, X; Ouyang, H
Journal of cellular and molecular medicine  18  326-43  2014

Afficher le résumé 		ImmunocytochemistryHuman24373095 24373095