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UFC910024 Amicon Ultra-15, PLHK, membrane Ultracel-PL, 100 kD

Ultracel-100 regenerated cellulose membrane, 15 mL sample volume

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Principales applications: Extraction de protéines, Purification de protéines
UFC910024
24  
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Aperçu

Replacement Information

Tableau de caractéristiques principal

Key ApplicationsNMWLVolumeVolume minimal du concentrat final
Extraction de protéines, Purification de protéines100 KDa15 mL200 µL
Description
RéférenceUFC910024
Nom de marque
  • Amicon Ultra
DescriptionAmicon Ultra-15, PLHK, membrane Ultracel-PL, 100 kD
Informations généralesAMICON ULTRA-15 15ML - 100 KDa cutoff. Available in 3 pack sizes.
UFC910008 - 8 pack
UFC910024 - 24 pack
UFC910096 - 96 pack



High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
High retentate recovery of greater than 90%
Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
Heat-sealed membrane minimizes downstream extractables
100% integrity tested for reliable performance
Convenient sample monitoring with translucent housing and volume gradations
Direct pipettor sample access eliminates processing step to recover concentrate
High concentration factors of 80–100X

Applications:
Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
Concentration of dilute or pre-purified proteins from column eluents
Desalting, buffer exchange and protein dialysis
Références bibliographiques
Informations produit
Code du filtrePLHK
Code couleurTransparent avec bouchon rose
Type de filtreUltrafiltration
PMNL100 KDa
Quality LevelMQ400
Applications
ApplicationUltracel-100 regenerated cellulose membrane, 15 mL sample volume
Principales applications
  • Extraction de protéines
  • Purification de protéines
Notes opératoiresRotor à godets mobiles : 4000 x g ; Rotor à angle fixe (35°) : 5000 x g
Informations biologiques
StérilitéNon stérile
Informations physico-chimiques
Application de centrifugationConcentration et dessalage de protéines
Dimensions
Longueur12.1 cm (4.807 in.)
Diamètre2.97 cm
Surface de filtration7.6 cm²
Volume minimal du concentrat final200 µL
Volume15 mL
Informations sur les matériaux
Chimie
  • Regenerated Cellulose (RC)
Matériau du dispositif
  • Support : Styrène/butadiène
  • Tube : Polypropylène
  • Bouchon avec revêtement interne : Polyéthylène
Matériau du supportCellulose régénérée
Informations toxicologiques
Informations de sécurité selon le SGH
Informations sur la sécurité
Notifications sur l'utilisation du produit
Informations relatives au stockage et à l'expédition
Informations sur l'emballage
Quantité24
Informations sur le transport
Information complémentaire
Caractéristiques
Global Trade Item Number
Référence GTIN
UFC910024 04053252317439

Documentation

Références bibliographiques

Aperçu de la référence bibliographiqueNº PubMed
Specific Lipids Modulate the Transporter Associated with Antigen Processing (TAP).
Schölz, Christian, et al.
J. Biol. Chem., 286: 13346-56 (2011)  2010

Afficher le résumé 		21357424 21357424

Certificats de qualité

Titre
UFC910024 - 0000241948

Brochure

Titre
REFINE PROTEIN PREPARATION. Tools for better protein analysis

FAQ

QuestionRéponse
Amicon Ultra units are offered in what volumes?Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
What is the recommended rotor for Amicon Ultra units?Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra?Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.
Which membranes are available in Amicon Ultra units?Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO.
Can the Amicon Ultra be spun to dryness?No, the Amicon Ultra has a built in deadstop.
Can the Amicon Ultra be used with nucleic acids?Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.
What is the maximum sample volume for Amicon Ultra?
Amicon Ultra-4Amicon Ultra-15
Swinging Bucket4ml 15ml
Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
3.5ml(23 degree rotor angle)
What is the chemical compatibility of Amicon Ultra?Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support.
Can Amicon Ultra units be sterilized with ethanol?Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization.
Do you have any centrifugal filter units that are pyrogen free?We do not have any centrifugals devices that we claim are pyrogen free. Even the sterile devices could contain pyrogens.
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Manuels d'utilisation

Titre
Amicon® Ultra-15 Centrifugal Filter Devices Non IVD User guide