Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

110630 AFB-Color Vert malachite (oxalate) solution de coloration

for the microscopic investigation of acid-fast bacteria (AFB) (cold staining)

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
110630
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
1106300500 500 ml
Description
Référence110630
DescriptionAFB-Color malachite green (oxalate) solution
AperçuAFB-Color malachite green (oxalate) solution - for the microscopic investigation of acid-fast bacteria (AFB) (cold staining), is a ready-to-use solution used for human-medical cell diagnosis and serves the purpose of the bacteriological investigation of sample material of human origin., for example smears of enriched bacterial cultures. Acid-fast bacteria are difficult to stain because of the high proportion of lipid and wax in their cell walls. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus the formation of the mycolate-fuchsin complex in the cell wall. In this case, a modification of the AFB-Color carbol fuchsin (Cat.no. 108512) solution renders heating unnecessary (Kinyoun method, cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use malachite green is used for counterstaining.
Pretreatment of the specimens with Sputofluol® (Cat. No. 108000) dissolves the bacteria from the surrounding viscid sputum and cell material.
The 500 ml bottle of AFB-Color malachite green (oxalate) solution provides 250 stainings. It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Références bibliographiques
Informations produit
Code SH3212 90 00
Quality LevelMQ400
Applications
Applicationfor the microscopic investigation of acid-fast bacteria (AFB) (cold staining)
Informations biologiques
Informations physico-chimiques
Densité1.0 g/cm3 (20 °C)
Dimensions
Informations sur les matériaux
Informations toxicologiques
Informations de sécurité selon le SGH
Classe de stockage10 - 13 Autres liquides ou matières solides
WGKWGK 2 Mise en danger significative de l'eau
Élimination28
Solutions aqueuses: récipient D.
Informations sur la sécurité
Notifications sur l'utilisation du produit
Informations relatives au stockage et à l'expédition
StockageConserver de +15°C à +25°C.
Informations sur l'emballage
Informations sur le transport
Information complémentaire
Caractéristiques
Suitability for microscopypasses test
Acid-fast bacteriared
Backgroundlight green
Global Trade Item Number
Référence GTIN
1106300500 04022536126357