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565758 β-Secretase Substrate IV, Fluorogenic - CAS 310427-94-2 - Calbiochem

β-Secretase Substrate IV, Fluorogenic, is a highly sensitive FRET substrate. Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: FS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic

565758
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Products

RéférenceConditionnement Qté
565758-1MG Ampoule plast. 1 mg
Description
OverviewA highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement. kcat/Km values (min-1/µM) at 37°C, pH 4.5, for pro-memapsin 2 and its activated species Leu28p-memapsin 2 are 0.044 and 0.040, respectively.
Catalogue Number565758
Brand Family Calbiochem®
SynonymsFS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic
References
ReferencesErmolieff, J., et al. 2000. Biochemistry 39, 12450.
Product Information
CAS number310427-94-2
ATP CompetitiveN
FormRed lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₉₁H₁₂₉N₂₅O₂₅S
Chemical formulaC₉₁H₁₂₉N₂₅O₂₅S
ReversibleN
Quality LevelMQ100
Applications
Applicationβ-Secretase Substrate IV, Fluorogenic, is a highly sensitive FRET substrate. Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement.
Biological Information
Primary Targetβ-secretase substrate
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceH-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
565758-1MG 04055977267709

Documentation

β-Secretase Substrate IV, Fluorogenic - CAS 310427-94-2 - Calbiochem FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

β-Secretase Substrate IV, Fluorogenic - CAS 310427-94-2 - Calbiochem Certificats d'analyse

TitreNuméro de lot
565758

Références bibliographiques

Aperçu de la référence bibliographique
Ermolieff, J., et al. 2000. Biochemistry 39, 12450.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-September-2008 RFH
SynonymsFS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic
DescriptionA highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm; emission max.: ~490), based on the GVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by β-secretase results in fluorescence enhancement. Reported Kcat/km values (min-1/µM) for pro-mempasin 2 and its proteolytically activated species Leu28p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37°C).
FormRed lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
CAS number310427-94-2
Chemical formulaC₉₁H₁₂₉N₂₅O₂₅S
Peptide SequenceH-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml)
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.
Toxicity Standard Handling
ReferencesErmolieff, J., et al. 2000. Biochemistry 39, 12450.