Typical Protein Recovery
Membrane NMWL, kDa Protein Solute Protein Retentate Volume, mL Protein Retentate Recovery, %
3 Cytochrome c, 12.4 kDa (0.25 mg/mL) 0.294 97.5
10 10 Cytochrome c, 12.4 kDa (0.25 mg/mL) 0.24 93.03
30 30 BSA, 67 kDa (1 mg/mL) 0.30 97.94
50 50 BSA, 67 kDa (1 mg/mL) 0.17 93.32
100 100 IgG, 156 kDa (1 mg/mL) 0.28 89.03
15 mL starting volume in swinging bucket rotor at 4000 x g at 25 °C. Spin times: 3K (40 min); 10K and 100K (20 min); 30K (10 min); 50K (15 min).