| Method | ISO 6579:2002; AMD 1:2007 |
FDA BAM Chapter 5 | FSIS MLG 4.05 |
|---|---|---|---|
| Pre-enrichment | BPW, 37 ± 1°C; 18 ± 2 h |
Lactose Broth / TSB / Universal Pre-enrichment Broth / BPW / …, 35°C; 24±2 h | BPW, 35°C; 21 ± 3 h |
| Selective enrichment | MKTTn, 37 ± 1°C; 24 ± 3 h and RVS, 41.5 ± 1°C; 24 ± 3 h or MSRV 41.5 ± 1°C, 24 ± 3 h (48 ± 3 h) |
Tetrathionate Broth 43 ± 0.2°C or 35 ± 2.0°C; 24 ± 2 h and RVS, 42 ± 0.2°C; 24 ± 2 h or Selenite Cystine Broth, 35°C, 24 ± 2h | RVS 42 ± 0.5°C; 22 ± 2 h and Tetrathionate Broth, 42 ± 0.5°C; 20-24 h |
| Plating | XLD agar + additional, 37°C; 24 ± 3 h / (48 ± 3 h) | BS agar, XLD agar and Hektoen Enteric agar, 35°C; 24 ± 2 h | BGS agar + XLT4-agar or DMLIA agar, 35 ± 2°C; 21 ± 3 h |
Salmonella Testing
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Salmonella spp. are one of the most common causes of food poisoning worldwide and have been isolated from most types of raw food (e.g. meats, eggs and plant products). Their high resistance to drying combined with a very high heat resistance once dried makes Salmonella a potential problem in most foods, particularly in dry and semi-dry products. Food legislation in many countries includes limits on Salmonella – typically absence in 25 g of food.
Merck maintains a comprehensive selection of products for the enrichment, detection and isolation of Salmonella. These include:
- Dehydrated Culture Media: Unique and convenient granulated media which meet the highest industry performance standards as set out in ISO 11133
- Our latest innovation: preweighed and gamma-irradiated Readybag® media pouches for quick and simple Salmonella testing
- Singlepath® Lateral Flow Tests: Innovative immunoassays in a “pregnancy test” format for detection with ultimate convenience in as little as 20 minutes after enrichment.
Salmonella is a genus of rod-shaped, Gram-negative, non-sporeforming, predominantly motile bacteria belonging to the Enterobacteriaceae family. Salmonella spp. are one of the most common causes of food poisoning worldwide and have been isolated from most types of raw food (e.g. meats, eggs and plant products). Their high resistance to drying combined with a very high heat resistance once dried makes Salmonella a potential problem in most foods, particularly in dry and semidry products.
Food legislation in many countries includes limits on Salmonella – typically absence in 25 g of food.
Salmonellosis
Most individuals infected with Salmonella develop diarrhea, fever, and abdominal cramps 12–72 hours after infection. The illness usually lasts 4–7 days, and the majority of patients recover without treatment. However, in some cases, the diarrhea may be so severe that hospitalization is required. In these patients, the Salmonella infection may spread from the intestines to the blood stream and then to other body sites. This can result in death unless the person is treated promptly with antibiotics. The elderly, infants, and those with impaired immune systems are more likely to have a severe illness.
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