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42600 Ultrafree-DA Centrifugal Filter Unit

Polypropylene, 0.5 mL volume, non-sterile, for DNA extraction

Ultrafree-DA Centrifugal Filter Unit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
42600
50  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number42600
Trade Name
  • Ultrafree
DescriptionUltrafree-DA Centrifugal Filter Unit
OverviewRecover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin. The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).

The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.

Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.

  • Minimal hands-on time
  • 10-minute spin
  • Fully-functional DNA


Performance
Typical DNA Recoveries from Agarose Gels

DNA Size (bp) Percent DNA Recoveries

100 78
700 71
1000 77
2027 47
4361 35
9416 32
23130 29

Protocol
1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.
2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.
3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.
4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.
References
Product Information
HS Code8421 29 90
Color CodeBlue
Maximum Operating Temperature50 °C
Filter TypeMicrofiltration
Quality LevelMQ400
Applications
ApplicationPolypropylene, 0.5 mL volume, non-sterile, for DNA extraction
Key Applications
  • DNA Extraction
Application NotesFixed-angle microfuge rotor w/maximum 5,000 x g
Biological Information
SterilityNon-Sterile
Physicochemical Information
Pore SizeNot Applicable
Centrifugal ApplicationDNA Gel Extraction
Dimensions
Length45 mm
Diameter10.7 mm
Filtration Area0.2 cm²
Volume0.5 mL
Materials Information
Chemistry
  • Polypropylene (PP)
Device Material
  • Polypropylene
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Packaging Information
Material Size50
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
42600 04053252743214

Related Products & Applications

Related Products

Catalogue Number Description
LSKGEL050 Montage Gel Extraction Kit
LSKMTAE50 Modified TAE Buffer 50x concentration, 500 mL

Product Families

DNA Extraction from Agarose Gels

Recover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin.
Learn More >>

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Ultrafree®

Categories

Life Science Research > Genomic Analysis > DNA Preparation & Cloning > DNA Purification > Gel Extraction Centrifugal Filter Units