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69048 T7•Tag® Antibody HRP Conjugate

T7•Tag® Antibody HRP Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69048
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69048-3 Plastic ampoule 100 ul
Description
OverviewThe T7•Tag Antibody HRP Conjugate is useful as a single detection reagent and eliminates the need for secondary antibodies or streptavidin conjugates.The enzyme conjugated T7•Tag monoclonal antibody enables detection with only one binding step and eliminates cross-reactivity associated with secondary reagents.

The T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors).

This antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase.

SpecificityT7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
Species/IsotypeMouse monoclonal IgG2b
Cross-reactivityNegligible with bacterial, insect or mammalian cell lysates
Sensitivity1–10 ng (Western blot developed with chromogenic substrates)
< 1 ng (developed with chemiluminescent substrates)
FormStabilized solution of antibody conjugate in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
Working dilution1:5,000–1:10,000
Catalogue Number69048
Brand Family Novagen®
References
Product Information
Components
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69048-3 04055977259780

Documentation

T7•Tag® Antibody HRP Conjugate Certificates of Analysis

TitleLot Number
69048

Citations

Title
  • Niharika B. Mettu, et al. (2007) The nuclear receptor-coactivator interaction surface as a target for peptide antagonists of the peroxisome proliferator-activated receptors. Molecular Endocrinology 21, 2361-2377.
  • Bryan M. Wittmann, Andrea Sherk and Donald P. McDonnell. (2007) Definition of functionally important mechanistic differences among selective estrogen receptor down-regulators. Cancer Research 67, 9549-9560.
  • Debaditya Mukhopadhyay, et al. (2006) SUSP1 antagonizes formation of highly SUMO2/3-conjugated species. Journal of Cell Biology 174, 939-949.
  • Pierre Fotso, et al. (2005) Cog1p plays a central role in the organization of the yeast conserved oligomeric golgi (COG) complex. Journal of Biological Chemistry 280, 27613-27623.
  • Erika Isono, et al. (2005) Functional analysis of Rpn6p, a lid component of the 26S proteasome, using temperature-sensitive rpn6 mutants of the yeast Saccharomyces cerevisiae. Journal of Biological Chemistry 280, 6537-6547.
  • Yoshimitsu Takahashi and Yoshiko Kikuchi. (2005) Yeast PIAS-type Ull1/Siz1 is composed of SUMO ligase and regulatory domains. Journal of Biological Chemistry 280, 35822-35828.
  • Naohide Watanabe and Eric Lam. (2005) Two Arabidopsis metacaspases AtMCP1b and AtMCP2b are arginine/lysine-specific cysteine proteases and activate apoptosis-like cell death in yeast. Journal of Biological Chemistry 280, 14691-14699.
  • Taruho S. Kuroda, Hiroyoshi Ariga and Mitsunori Fukuda. (2003) The actin-binding domain of Slac2-a/melanophilin is required for melanosome distribution in melanocytes. Molecular and Cellular Biology 23, 5245-5255.
  • Ken-Shwo Dai and Choong-Chin Liew. (2001) A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via Its RING domain. Journal of Biological Chemistry 276, 23992-23999.
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    User Protocols

    Title
    TB137 T7•Tag® Antibody HRP Conjugate