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GF128 Stromal Cell-Derived Factor-1α Protein, Recombinant mouse

Stromal Cell-Derived Factor-1 alpha protein (or SDF-1 alpha protein) is a recently discovered protein belonging to the alpha chemokine (CXC) family of cytokines.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: P48061, P40224
GF128
10 µg  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Key ApplicationsSpeciesSourcePurity
CULTMouse E.coliGreater than 99% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/ug) of SDF-1 alpha.
Description
Catalogue NumberGF128
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionStromal Cell-Derived Factor-1α Protein, Recombinant mouse
OverviewStromal Cell-Derived Factor-1 alpha (SDF-1 alpha) is a recently discovered protein belonging to the alpha chemokine (CXC) family of cytokines. Recombinant Mouse SDF-1 alpha is a 7.9 kDa protein containing 68 amino acid residues.
Alternate Names
  • SDF-1alpha
Product Information
PresentationThe sterile filtered solution was lyophilized with no additives.
Quality LevelMQ100
Applications
ApplicationStromal Cell-Derived Factor-1 alpha protein (or SDF-1 alpha protein) is a recently discovered protein belonging to the alpha chemokine (CXC) family of cytokines.
Key Applications
  • Cell Culture
Biological Information
PurityGreater than 99% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/ug) of SDF-1 alpha.
SourceE.coli
Specific ActivityDetermined by its ability to chemoattract human peripheral blood monocytes using a concentration of 50-100ng/ml.
Entrez Gene SummaryFor background information on chemokines, see CXCL11 (SCYB11; MIM 604852). Stromal cell-derived factors 1-alpha and 1-beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF (see MIM 191160), or IL1 (see MIM 147760). The intercrines are characterized by the presence of 4 conserved cysteines which form 2 disulfide bonds. They can be classified into 2 subfamilies. In the CC subfamily, which includes beta chemokine, the cysteine residues are adjacent to each other. In the CXC subfamily, which includes alpha chemokine, they are separated by an intervening amino acid. The SDF1 proteins belong to the latter group.[supplied by OMIM]
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P48061 # Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites.
SIZE: 93 amino acids; 10666 Da
SUBCELLULAR LOCATION: Secreted.
PTM: Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processsed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
SIMILARITY: SwissProt: P48061 ## Belongs to the intercrine alpha (chemokine CxC) family.
Stem Cell Type
  • Hematopoietic Stem Cells
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Reconstituted mouse SDF-1 alpha should be stored in undiluted aliquots at -20°C. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size10 µg
Global Trade Item Number
Catalogue Number GTIN
GF128 04053252272264