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SRI-13K Sensitive Rat Insulin RIA

SRI-13K
250 tubes  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Insulin RRIARadioactive 125I
Description
Catalogue NumberSRI-13K
DescriptionSensitive Rat Insulin RIA
Background InformationInsulin, a 5.8 kDa hormone is secreted by the islet beta cells in the pancreas. This kit offers sensitive assay for rat/mouse insulin
Product Information
Detection methodRadioactive 125I
Performance
  • ED80 = 0.09 ± 0.01 ng/mL
  • ED50 = 0.22 ± 0.01 ng/mL
  • ED20 = 0.55 ± 0.01 ng/mL
Precision, %
  • Inter-assay: 3.8–10.8
  • Intra-assay: 2.7–5.8
Quality LevelMQ100
Applications
ApplicationThis radioimmunoassay (RIA) measures & quantifies Insulin for use in Metabolism & Endocrine research.
Key Applications
  • Radioimmunoassay
Application NotesTwo-day disequilibrium incubation at 4°C. Sample volume 100 μL serum, plasma, or tissue culture media.
Biological Information
SpecificityRat Insulin I 100% Rat Insulin II 100% Human Insulin 100% Porcine Insulin 100% Sheep Insulin 100% Hamster Insulin 100% Mouse Insulin 100% Rat C-Peptide Not Detectable Glucagon Not Detectable Somatostatin Not Detectable Pancreatic Polypeptide No
Species Reactivity
  • Rat
Analytes Available
  • Insulin
Radioactive Isotype125I-Insulin
Accuracy
  • 88–108%
Physicochemical Information
Linearity of Dilution84–119%
Standard Curve Range
  • 0.02–1.0 ng/mL
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size250 tubes
Global Trade Item Number
Catalogue Number GTIN
SRI-13K 04053252727061